Study of RASSF1A expression and promoter demethylation in Hep-2 cell line / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
; (24): 64-66, 2011.
Article
em Zh
| WPRIM
| ID: wpr-747426
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the effect of 5-Aza-dC and TSA to tumor suppressor gene RASSF1A expression and methylation level in Hep-2 cell line.@*METHOD@#Hep-2 cell line were cultured in vitro and handled with 5-Aza-dC and TSA. Detected RASSF1A expression and methylation level were detected before and after drug intervention using Realtime PCR and MSP.@*RESULT@#(1) Before intervention with drug, tumor suppressor gene RASSF1A was weakly expressed and methylated in Hep-2 cell line. (2) With the effect of 5-Aza-dC and TSA, the methylation of RASSF1A gene was reversed. And the effect of combination of 5-Aza-dC and TSA was similar with 5-Aza-dC alone. There was no obvious effect using TSA alone. (3) With the effect of 5-Aza-dC and TSA, the expression of RASSF1A was improved. And the effect of 5-Aza-dC was stronger than TSA. Synergetic effect was found when using 5-Aza-dC and TSA simultaneously.@*CONCLUSION@#In Hep-2 cell line, Promoter methylation of tumor suppressor RASSF1A may play a very important role in loss of gene expression, but it is not the only cause. 5-Aza-dC and TSA can improve RASSF1A expression by reversing DNA methylation and histone deacetylation.
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Base de dados:
WPRIM
Assunto principal:
Farmacologia
/
Azacitidina
/
Expressão Gênica
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Genes Supressores de Tumor
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Regiões Promotoras Genéticas
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Metilação de DNA
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Inativação Gênica
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Proteínas Supressoras de Tumor
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Linhagem Celular Tumoral
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Genética
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Ano de publicação:
2011
Tipo de documento:
Article