Bile Acid Transporters Are Expressed and Heterogeneously Distributed in Rat Bile Ducts
Gut and Liver
; : 569-575, 2019.
Article
em En
| WPRIM
| ID: wpr-763869
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND/AIMS: Cholangiocytes are capable of reabsorbing bile salts from bile, but the pathophysiological significance of this process is unclear. To this end, we detected the expression and distribution of bile acid transport proteins in cholangiocytes from normal rat liver and analyzed the possible pathophysiological significance. METHODS: Bile duct tissues of Sprague-Dawley rats were isolated by enzymatic digestion and mechanical isolation, and then divided into large and small bile duct tissues. Immunohistochemistry, real-time polymerase chain reaction and Western blotting were used to determine the expression of the apical sodium-dependent bile acid transporter (ASBT), ileal bile acid binding protein (IBABP), and basolateral organic solute transporter α (Ostα) in the biliary tract system of rats. Differences in the expression and distribution of these proteins were analyzed. RESULTS: In cholangiocytes, ASBT and IBABP were mainly expressed in cholangiocytes of the large bile ducts, in which the expression of both was significantly higher than that in the small ducts (p0.05). CONCLUSIONS: Bile acid transporters are expressed and heterogeneously distributed in rat bile ducts, indicating that bile acid reabsorption by cholangiocytes might mainly occur in the large bile ducts. These findings may help explore the physiology of bile ducts and the pathogenesis of various cholangiopathies.
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Base de dados:
WPRIM
Assunto principal:
Fisiologia
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Características da População
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Bile
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Ductos Biliares
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Sistema Biliar
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Ácidos e Sais Biliares
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Imuno-Histoquímica
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Proteínas de Transporte
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Western Blotting
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Ratos Sprague-Dawley
Aspecto:
Determinantes_sociais_saude
Limite:
Animals
Idioma:
En
Revista:
Gut and Liver
Ano de publicação:
2019
Tipo de documento:
Article