Neutrophilic Granule Protein Is a Novel Murine LPS Antagonist
Immune Network
; : e34-2019.
Article
em En
| WPRIM
| ID: wpr-764026
Biblioteca responsável:
WPRO
ABSTRACT
Neutrophilic granule protein (NGP) was previously reported as a granular protein of neutrophils in mouse, but the function has not been known clearly. We found the presence of the possible signal peptide in NGP and validated this protein is circulating in the bloodstream. In our findings, NGP is being modified post-translationally in Golgi apparatus and endoplasmic reticulum, which is a universal character of secretory molecules with a signal peptide. The secreted NGP protein could be detected both in vitro and in vivo. NGP has sequence similarity with an antimicrobial protein cathelicidin, and we observed the aspect of inflammation of NGP. Interestingly, NGP interacts with the complex of LPS and LPS binding protein (LBP). This interaction blocks the binding of the complex of LPS and LBP to TLR4 and the downstream inflammatory signals. Furthermore, the inhibitory function of NGP against the inflammatory effect of LPS could be observed in both in vitro and in vivo. With these findings, we report NGP is a novel secretory protein to mask LPS and inhibit its function.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Ligação Proteica
/
Técnicas In Vitro
/
Sinais Direcionadores de Proteínas
/
Proteínas de Transporte
/
Lipopolissacarídeos
/
Citocinas
/
Retículo Endoplasmático
/
Complexo de Golgi
/
Inflamação
/
Máscaras
Limite:
Animals
Idioma:
En
Revista:
Immune Network
Ano de publicação:
2019
Tipo de documento:
Article