Induction of Apoptosis Scutellaria baicalensis Georgi Root Extract by Inactivation of the Phosphatidyl Inositol 3-kinase/Akt Signaling Pathway in Human Leukemia U937 Cells
Journal of Cancer Prevention
; : 11-19, 2019.
Article
em En
| WPRIM
| ID: wpr-764300
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND:
The roots of Scutellaria baicalensis Georgi (Labiatae) have been widely used in traditional medicine for treatment of various diseases. In this study, we investigated the effects of ethanol extracts of S. baicalensis roots (EESB) on the growth ofn human leukemia U937 cells.METHODS:
The effect of EESB on cell viability was measured by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. Apoptosis was determined using 4,6-diamidino-2-phenyllindile staining and flow cytometry. The effects of EESB on the expression of regulatory proteins of apoptosis and phosphatidyl inositol 3-kinase (PI3K)/Akt signaling were determined by Western blotting. Caspase activity and mitochondrial membrane potential (MMP) were measured using flow cytometric analysis.Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Fosfatidilinositóis
/
Leucemia
/
Regulação para Baixo
/
Regulação para Cima
/
Sobrevivência Celular
/
Western Blotting
/
Apoptose
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Células U937
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Scutellaria
/
Scutellaria baicalensis
Limite:
Humans
Idioma:
En
Revista:
Journal of Cancer Prevention
Ano de publicação:
2019
Tipo de documento:
Article