Esculetin Prevents the Induction of Matrix Metalloproteinase-1 by Hydrogen Peroxide in Skin Keratinocytes
Journal of Cancer Prevention
; : 123-128, 2019.
Article
em En
| WPRIM
| ID: wpr-764303
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND:
Reactive oxygen species (ROS) are involved in various cellular diseases. Excessive ROS can cause intracellular oxidative stress, resulting in a calcium imbalance and even aging. In this study, we evaluated the protective effect of esculetin on oxidative stress-induced aging in human HaCaT keratinocytes.METHODS:
Human keratinocytes were pretreated with esculetin for 30 minutes and treated with H₂O₂. Then, the protective effects on oxidative stress-induced matrix metalloproteinase (MMP)-1 were detected by Flou-4-AM staining, reverse transcription-PCR, Western blotting, and quantitative fluorescence assay.RESULTS:
Esculetin prevented H₂O₂-induced aging by inhibiting MMP-1 mRNA, protein, and activity levels. In addition, esculetin decreased abnormal levels of phospho-MEK1, phospho-ERK1/2, phospho-SEK1, phospho-JNK1/2, c-Fos, and phospho-c-Jun and inhibited activator protein 1 binding activity.CONCLUSIONS:
Esculetin prevented excessive levels of intracellular calcium and reduced the expression levels of aging-related proteins.Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Pele
/
Envelhecimento
/
RNA Mensageiro
/
Queratinócitos
/
Western Blotting
/
Cálcio
/
Espécies Reativas de Oxigênio
/
Fator de Transcrição AP-1
/
Estresse Oxidativo
/
Metaloproteinase 1 da Matriz
Limite:
Humans
Idioma:
En
Revista:
Journal of Cancer Prevention
Ano de publicação:
2019
Tipo de documento:
Article