Sequencing of a large off-ladder allele at Penta E locus / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 168-170, 2019.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-775786
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To identify a rare large off ladder (OL) allele at the Penta E locus.@*METHODS@#Chelex-100 was used to extract DNAs from the blood samples. The PCR fragments were purified, extracted, and subjected to TA cloning and sequencing.@*RESULTS@#An OL allele was identified by the PowerPlex™ 21 at the Penta E locus, which was postulated as allele 26 based on assigned size. The OL allele was verified as a novel fragment containing 26 full AAAGA repeats.@*CONCLUSION@#OL alleles and microvariants should be verified by direct sequencing. Typing of OL alleles has significance for both daily work and forensic genetics.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Reação em Cadeia da Polimerase
/
Rubiaceae
/
Alelos
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2019
Tipo de documento:
Artigo