Lab-on-chip for the detection of food borne pathogenic bacteria / 上海预防医学
Shanghai Journal of Preventive Medicine
; (12): 330-334, 2014.
Article
em Zh
| WPRIM
| ID: wpr-789281
Biblioteca responsável:
WPRO
ABSTRACT
Objective To set up an onsite protocol for abrupt emergencies of public health e-vents, with both the sensitivity and accuracy of the lab-on-chip(microfluidic chip), for the detection of food borne pathogenic bacterium including Vibrio cholera , Salmonella , Vibrio parahaemolyticus and Shigellaare exanimated . [ Methods] By comparison of the results from the chips and fluorescence quantitative PCR were analyzed the specificity , sensitivity, accuracy and repeatability of the Lab-on-chip. [ Results] Acceptable specificity , accuracy and repeatability of the chips had been well achieved , the detection limit of the chips for the Vibrio cholera , Salmonella, Vibrio parahaemolyticus and Shigella was 5 ×103 ~103 DNA Copies/mL. [ Conclusion] Lab-on-chip is believed to be a fast , convenient , efficient tool with accept-able accuracy for public health emergencies .
Texto completo:
1
Base de dados:
WPRIM
Tipo de estudo:
Diagnostic_studies
Idioma:
Zh
Revista:
Shanghai Journal of Preventive Medicine
Ano de publicação:
2014
Tipo de documento:
Article