Comparative study of quercetin and allopurinol on serum uric acid levels and function of liver and kidney in hyperuricemic rats / 中国药科大学学报

ABSTRACT

@#To investigate the therapeutic effect and influence of quercetin and allopurinol on the function of liver and kidney in hyperuricemic rats, male SD rats were administered with the drugs by oral gavage once a day for seven consecutive days throughout the experiment. On the fifth day, the animal model of hyperuricemia was set up by hypoxanthine intraperitoneal injection. The serum was used for assaying the uric acid(UA), alanine aminotransferase(ALT), aspartate aminotransferase(AST), total bilirubin(TBIL), direct bilirubin(DBIL), β2-microglobulin(β2-MG), serum cystatin C(Cys-C), urea nitrogen(Urea)and serum creatinine(Cr)with colorimetry, continuous monitoring, chemical oxidation and enzyme-linked immunosorbent assay. The results showed that quercetin had no effect on the uric acid levels of serum, and that allopurinol reduced uric acid levels in rats significantly(P< 0. 01). The serum levels of AST and ALT in rats substantially decreased compared with normal control group(P< 0. 01), while no significant differences were found in TBIL, and DBIL. Compared with normal control group, β2-MG and Cys-C levels were significantly increased in the other five groups(P< 0. 01), serum levels of Urea and Cr were significantly higher in the allopurinol group compared to the normal control group(P< 0. 01). Modeling and administration group showed mild histopathological changes in rat kidney. The results clearly demonstrated that quercetin had no effect on the uric acid levels of serum, and allopurinol lowered uric acid significantly. There was no significant effect on the function of liver by modeling and administration, however, there was potential impaiment of renal function after modeling. Quercetin did not exhibit a protective effect on renal injury and administration with allopurinol increased kidney damage.