Your browser doesn't support javascript.
loading
Integrating qualitative and quantitative characterization of Prunellae Spica by HPLC-QTOF/MS and HPLC-ELSD / 中国天然药物
Article em En | WPRIM | ID: wpr-812599
Biblioteca responsável: WPRO
ABSTRACT
The present study was designed to analyze the major constituents in Prunellae Spica and establish a method for simultaneous determination of two constituents contained in Prunellae Spica. High performance liquid chromatography coupled with time-of-flight mass spectrometry (HPLC-QTOF-MS/MS) technique was used to identify the constituents in the extractive of Prunellae Spica. High performance liquid chromatography coupled with evaporative light scattering detection (HPLC-ELSD) was used to simultaneously quantify two kinds of constituents contained in Prunellae Spica. Principal component analysis (PCA) was applied to compare the similarity and difference among samples from different regions of China. In the present study, 22 compounds were identified and some new fragmental pathways of triterpenic acids were discovered. An accurate and reliable HPLC-ELSD method was developed and validated for the first time to simultaneously quantify multiple constituents, including rosmarinic acid, maslinic acid, corosolic acid, betulin, oleanolic acid, and ursolic acid in the extract of Prunellae Spica. (PCA) revealed some similarities and differences among different samples from different regions of China. In conclusion, our results from this study would be helpful in establishing a scientific and rational quality control method for Prunellae Spica.
Assuntos
Palavras-chave
Texto completo: 1 Base de dados: WPRIM Assunto principal: Triterpenos / Medicamentos de Ervas Chinesas / Estrutura Molecular / Química / China / Cromatografia Líquida de Alta Pressão / Cinamatos / Prunella / Depsídeos / Espectrometria de Massas em Tandem Tipo de estudo: Qualitative_research País/Região como assunto: Asia Idioma: En Revista: Chinese Journal of Natural Medicines (English Ed.) Ano de publicação: 2016 Tipo de documento: Article
Texto completo: 1 Base de dados: WPRIM Assunto principal: Triterpenos / Medicamentos de Ervas Chinesas / Estrutura Molecular / Química / China / Cromatografia Líquida de Alta Pressão / Cinamatos / Prunella / Depsídeos / Espectrometria de Massas em Tandem Tipo de estudo: Qualitative_research País/Região como assunto: Asia Idioma: En Revista: Chinese Journal of Natural Medicines (English Ed.) Ano de publicação: 2016 Tipo de documento: Article