SARA expression in high glucose induced epithelium to mesenchymal transition of proximal tubule cells / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 1230-1235, 2010.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-814340
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To construct the cell model of epithelium to mesenchymal transition of proximal tubule cells induced by high glucose and to determine the expression of Smad anchor for receptor activation (SARA).@*METHODS@#Protein expression of vimentin, Zona occludens-1(ZO-1), and SARA was determined by Western blot, and their mRNA expressions were detected by Real-time PCR.@*RESULTS@#After stimulation by 30 mmol/L D-glucose, the protein and mRNA expression levels of vimentin in HK-2 cells increased in a time-dependent manner while the expression of ZO-1 was reduced significantly, especially at 48 h. Meanwhile, SARA was also decreased in a time-dependent manner.@*CONCLUSION@#High glucose can induce renal epithelium to mesenchymal transition, and SARA may be involved in this process as a protector.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fosfoproteínas
/
Vimentina
/
RNA Mensageiro
/
Serina Endopeptidases
/
Transdução de Sinais
/
Diferenciação Celular
/
Células Cultivadas
/
Biologia Celular
/
Peptídeos e Proteínas de Sinalização Intracelular
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2010
Tipo de documento:
Artigo