Mechanism of DADS in the bystander effect of HSV-tk/GCV suicide gene therapy system in lens epithelial cells / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 329-334, 2011.
Article
em Zh
| WPRIM
| ID: wpr-814571
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WPRO
ABSTRACT
OBJECTIVE@#To investigate the mechanism and effect of diallyl disulfide (DADS) on the bystander effect of herpes simplex virus kinase/ganciclovir (HSV-tk/GCV) suicide gene therapy system which was mediated by recombinant adeno-associated virus (rAAV) in lens epithelial cells.@*METHODS@#Immunohistochemistry method was used to determine the distribution of connexin 43 (Cx43) protein in cultured rabbit lens epithelial cells and rabbit lens epithelial cells transfected by HSV-tk suicide gene. Cx43 protein was measured and analyzed in cultured rabbit lens epithelial cells and rabbit lens epithelial cells transfected by HSV-tk suicide gene that was induced by various DADS. Cell survival was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method.@*RESULTS@#DADS increased the Cx43 protein expression in cultured rabbit lens epithelial cells and rabbit lens epithelial cells transfected by HSV-tk suicide gene, and especially in 50 μmol/L DADS. After combining with DADS, the bystander effect was significantly improved (P<0.05).@*CONCLUSION@#DADS can elevate the expression of Cx43 protein and enhance the bystander effect of HSV-tk/GCV suicide gene therapy system.
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Base de dados:
WPRIM
Assunto principal:
Antivirais
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Farmacologia
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Timidina Quinase
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Óleos de Plantas
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Ganciclovir
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Células Cultivadas
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Adenoviridae
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Simplexvirus
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Conexina 43
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Biologia Celular
Limite:
Animals
Idioma:
Zh
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2011
Tipo de documento:
Article