Content Determination of 4 Indicator Components in Shengyu Decoction Lyophilized Powder by HPLC / 中国药房
China Pharmacy
; (12): 576-580, 2020.
Article
em Zh
| WPRIM
| ID: wpr-817313
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE:To establish the content determin ation method of ferulic acid ,verbascoside,ligustilide and astragaloside in Shengyu decoction lyophilized powder. METHODS :HPLC method was adopted to determine 4 components in 3 batches of lyophilized powder. The determination of ferulic acid ,verbascoside and ligustilide was performed on Inertsil ODS-SP C 18 column with mobile phase consisted of methanol- 0.1% phosphoric acid (gradient elution )at the flow rate of 1.0 mL/min;detector was diode array detector ;detection wavelength was set at 330 nm;column temperature was 30 ℃,the sample size was 10 μL. The determination of astragaloside was performed on Kromasil C 18 column with mobile phase consisted of acetonitrile-water (32∶68,V/ V);detector was evaporative light scattering detector ;the drift tube temperature wa s 100 ℃,the carrier gas (air)flow rate was 2.5 L/min at the flow rate of 1.0 mL/min;column temperature was 30 ℃,the sample size was 10 μL. RESULTS:The linear ranges of ferulic acid ,verbascoside,ligustilide and astragaloside were 0.050 15-10.03 μg(r=0.999 8),0.067 80-13.56 μg(r= 0.999 9),0.057 30-11.46 μg(r=0.999 5),1.128-11.28 μg(r=0.999 3),respectively. The detection limits were 2.12×10-4,1.30× 10-3,8.02×10-4,1.09×10-3 μg,respectively. The limit of quantification were 7.43×10-4,3.87×10-3,2.34×10-3,3.36×10-3 μg, respectively. RSDs of precision ,stability(12 h)and reproducibility tests were all lower than 2%(n=6). Average recovery rates were 99.6%(RSD=0.83%,n=6),100.9%(RSD=1.07%,n=6),98.8%(RSD=0.84%,n=6)and 101.3%(RSD=0.99%, n=6),respectively. The contents of ferulic acid ,verbascoside,ligustilide and astragaloside in 3 batches of samples were 1.225-1.248, 0.413-0.424, 0.325-0.332, 0.394-0.404 mg/g, respectively (RSDs among batches were lower than 1.5% ). CONCLUSIONS:Established method is stable ,reproducible,rapid and accurate for the content determination of ferulic acid , verbascoside, ligustilide and astragaloside in Shengyu
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
China Pharmacy
Ano de publicação:
2020
Tipo de documento:
Article