Expression of HIP/PAP in hepatocellular carcinoma and effect of siRNA on migration and invasion in HCC cells
Asian Pacific Journal of Tropical Medicine
; (12): 848-854, 2015.
Article
em En
| WPRIM
| ID: wpr-820462
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the expression of HIP/PAP in hepatocellular carcinoma (HCC) patients and explore its role in migration and invasion of HCC.@*METHODS@#The expression of HIP/PAP in HCC tissue and corresponding adjacent noncancerous tissue was assessed by IHC, RT-PCR and Western blot. The correlation between clinicopathological features and HIP/PAP expression was analyzed. The role of HIP/PAP on invasion and migration of HCC cells was observed by RNA interference, wound healing and Transwell assay.@*RESULTS@#Both mRNA and protein expression of HIP/PAP was upregulated in HCC tissues compared to tumor-adjacent tissue and correlated with poor tumor differentiation, advanced tumor stage and vascular invasion. HIP/PAP expression was also upregulated in HCC cells, and silencing its expression by specific siRNA could inhibit the invasion and migration of HCC cells.@*CONCLUSIONS@#HIP/APA is overexpressed in HCC and contributes to the migration and invasion of HCC cells.
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Base de dados:
WPRIM
Idioma:
En
Revista:
Asian Pacific Journal of Tropical Medicine
Ano de publicação:
2015
Tipo de documento:
Article