Effect and mechanism of metformin on TGF-β1expression in pancreatic cancer / 西安交通大学学报(医学版)
Journal of Xi'an Jiaotong University(Medical Sciences)
; (6): 506-511, 2019.
Article
em Zh
| WPRIM
| ID: wpr-843989
Biblioteca responsável:
WPRO
ABSTRACT
Objective: To investigate the effect of metformin on TGF-β1 expression in pancreatic cancer and further analyze the underlying mechanism. Methods: After metformin intervention, cell proliferation and invasion ability were detected by MTT assay and Transwell method, respectively. The expression of TGF-β1 in pancreatic cancer cell lines Panc-1 and BxPC-3 was detected by qRT-PCR, Western blot and immunofluorescence. The expression of AMPK/p-AMPK was detected by Western blot; ELISA method was used to measure the content of TGF-β1 in cell supernatant. After silencing AMPK expression in pancreatic cancer cell lines, the cells were treated with metformin and the expression of TGF-β1 was detected by qRT-PCR and Western blot. Results: The phosphorylation level of AMPK in Panc-1 (P<0.001) and BxPC-3 (P<0.001) cells was significantly decreased after metformin intervention. In addition, the expression of TGF-β1 in tumor cells was significantly decreased. However, the reduced expression of TGF-β1 by metformin was reversed by AMPK silencing in cancer cells (P<0.05). Conclusion: Metformin can inhibit TGF-β1 expression in pancreatic cancer cells by inducing AMPK phosphorylation.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Journal of Xi'an Jiaotong University(Medical Sciences)
Ano de publicação:
2019
Tipo de documento:
Article