Content comparison on quercetin from different parts and HPLC fingerprint of Lespedeza cuneata (Dum.cours.) G.Don / 国际药学研究杂志

ABSTRACT

Objective To establish a HPLC method for the determination of quercetin from different parts of L. cuncata(Dum. Cours.) G.Don., including roots, old branches, young shoots, leaves and seeds, and to build the fingerprints. Methods The HPLC method determination of quercetin and fingerpints were establised. Results The restults showed that there were great differences between the quercetin contents from different parts, with the highest contents found in seeds, followed by young shoots, leaves, old branches, and roots. The similarities of HPLC fingerprints of the medicinal material were quite different from the parts of L. cuncata (Dum.Cours.) G.Don.. The similarities were all above 0.95 for L. cuncata (Dum.Cours.) G.Don. samples, roots, old branches and young shoots below 0.85, leaves and seeds similarities below 0.60. Conclusion It was concluded that different parts (such as roots, old branches, young shoots, leaves, seeds) of L. cuncata(Dum.Cours.) G.Don. should be divided in clinical and productive practice so as to supply the scientific basis for enhancing the curative effect and reasonable utilization of the resource of L. cuncata(Dum.Cours.)G. Don.