Feasibility of replacing cholesterol by β-sitosterol to prepare quercetin liposomes / 中草药

ABSTRACT

Objective: To study the feasibility of preparing quercetin liposomes with β-sitosterol instead of cholesterol and optimize the prescription and preparation of quercetin liposomes. Methods: Quercetin liposomes were prepared by film dispersion method with lecithin and β-sitosterol, encapsulation efficiency (EE) was determined by centrifugal precipitation method. EE and drug loading were adopted to evaluate and optimize preparation process as response in orthogonal design. Quercetin liposomes were characterized by particle size, potential, atomic force microscope (AFM) and in vitro release. Results: Film dispersion method was the best method to prepare quercetin liposomes with lecithin and β-sitosterol. The optimum preparation process determined by orthogonal design was as follow the ratio of quercetin to lecithin was 15, the ratio of lecithin to β-sitosterol was 61, pH value of PBS buffer solution was 6.0, the hydration time was 2 h, the EE was (82.55 ± 1.10)%, drug loading was (11.40 ± 1.14)%, particle size was (194.60 ± 4.38) nm, Zeta potential was (-30.45 ± 0.42) mV, and the prepared quercetin liposomes were verified by AFM with round shape and homogenious particle size. There was no difference between new type liposome and ordinary liposome. Conclusion: Quercetin liposomes prepared with lecithin and β-sitosterol are practicable. The quercetin liposomes prepared by optimum preparation process have a high EE, good morphology, and reproducibility, and it can be used in industry.