Effects of gastrodine on expression of inflammatory cytokines in nerve microglia cell induced by advanced glycation end products / 中草药

ABSTRACT

Objective: To investigate the effects of gastrodine (GAS) on the expression of IL-Iβ and IL-6 in nerve microglia BV-2 cell of mice induced by different concentrations of advanced glycation end products (AGEs) and GAS intervention. Methods: The cultured BV-2 cells of mice were divided into AGEs group, GAS (12.5, 25, 50, and 100 mg/L) groups, which were cultured for 18 h to observe the changes of cell morphology. The levels of IL-1β and IL-6 in the cell culture supernate were determined by enzyme linked immunosorbent assay (ELISA) and the expressions of IL-1β and mRNA were analysed by reverse transcriptase polymerase chain reaction (RT-PCR). Results: Observed under an inverted microscope, microglia is mostly quiescent and less protruding in control group; Most cell processes were more and amoeba-like in AGEs group, which was most obviously at the concentration of 300 mg/L. Cell processes in GAS groups reduced less than those in AGEs groups, of which the least was in GAS group at concentration of 50 mg/L. Compared with the control group, the levels of IL-1β and IL-6 in supernatants of cell culture in AGEs group and the expressions of IL-1β and IL-6 mRNA increased with significant difference (P<0.05). Compared with AGEs groups, the levels of IL-1β and IL-6 in supernatants of cell culture in AGEs group and the expressions of IL-1β and IL-6 mRNA obviously decreased, there were significant differences in AGE groups at the concentrations of 25, 50, and 100 mg/L (P<0.05), among which the decrease was most significant in the AGE group of 50 mg/L (P<0.01). Conclusion: AGEs could induce that nerve microglia produce IL-1β and IL-6 and GAS could partly inhibit the induction of AGEs on microglia.