Investigation of the Excretion of Seven Constituents of Inula cappaort in Rat Urine by UPLC-MS/MS / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish an UPLC-MS/MS method for the analysis of seven compounds of Inula cappaort in rat urine to study their excretion. METHODS: The urine samples in 0-2, 2-6, 6-12, 12-24, and 24-36 h were collected. Acquity UPLC BEH C18 column (2.1 mm×50 mm, 1.7 μm) was used and the column temperature was set at 45 ℃, the mobile phase was 0.1% formic acid acetonitrile -0.1% formic acid aqueous solution in a gradient elution mode and flow rate was 0.25 mL•min-1. The detection was carried out by a triple quadrupole linear ion trap mass spectrometer in positive and negative ion mode with an electrospray source. Multiple reactions monitoring (MRM) mode was employed. RESULTS: The calibration curves showed good linearity, with correlation coefficients of greater than 0.991 0 for all of the analytes within the concentration ranges. The intra-day and inter-day precisions (RSD) were all less than 15%. The extraction recoveries of the seven components were more than 84.41%, without obvious matrix effect, which met the requirements for analysis. CONCLUSION: The established method is simple, rapid, and sensitive. It can be applied in the excretion study of the seven components of Inula cappaort extract in rat urine. The urine excretion test showed that the prototype excretion rates are low in rats, and the cumulative excretion rates are all less than 5%.