Determination of four saccharides in Qingkailing injection by HPLC-ELSD / 中国药学杂志
Chinese Pharmaceutical Journal
; (24): 1183-1186, 2013.
Article
em Zh
| WPRIM
| ID: wpr-860310
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE:
To establish an HPLC-ELSD method to determine the contents of four saccharides in Qingkailing injection and carry out cluster analysis of the products from different manufactures.METHODS:
Chromatographic separation was performed on a Prevail Carbohydrate ES column with mobile phase of acetonitrile (B) - water (A) at a flow rate of 1.0 mL·min-1 with gradient elution; the column temperature was 30°C; the ELSD drift tube temperature was 90.7°C and the gas flow was 2.4 L·min-1. Cluster analysis was performed by SPSS16.0.RESULTS:
Good resolution and linearities were obtained. The average recoveries of xylose, fructose, glucose and sucrose were 98.52% (RSD=1.70%), 99.15% (RSD=2.55%), 99.82% (RSD=2.04%) and 99.26% (RSD=1.67%), respectively. The test samples were clustered into three classes.CONCLUSION:
The established method is simple, reliable and accurate. It can be used to determine the saccharide components in Qingkailing injection, and provide theoretical basis for its quality control.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Chinese Pharmaceutical Journal
Ano de publicação:
2013
Tipo de documento:
Article