Preparation of an anti-cotinine monoclonal antibody and its application in immunological detection / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 765-771, 2020.
Article
em Zh
| WPRIM
| ID: wpr-879938
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To prepare monoclonal antibody against cotinine (COT) and to establish immunoassay for detecting COT in human urinary samples.@*METHODS@#BALB/c mice were immunized with synthesized cotinine-bovine serum albumin (COT-BSA) to screen monoclonal antibody with technique of cell fusion. The monoclonal antibody was used for the indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and colloidal gold immunochromatographic strip assay for the detection of COT in human urine.@*RESULTS@#The monoclonal antibody against COT was identified by ic-ELISA with a 50%inhibitive concentration (IC@*CONCLUSIONS@#The ic-ELISA and colloidal gold immunochromatographic strip assay using the prepared monoclonal antibody against COT have been proved to be reliable for the rapid detection of COT in human urines, which may be used for monitoring of environmental tobacco smoke.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Ensaio de Imunoadsorção Enzimática
/
Urinálise
/
Coloide de Ouro
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Cotinina
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Camundongos Endogâmicos BALB C
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Anticorpos Monoclonais
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
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Humans
Idioma:
Zh
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2020
Tipo de documento:
Article
País de publicação:
CHINA
/
CN
/
REPUBLIC OF CHINA