Generation and phenotypic characterization of S100A9 gene knockout mice by CRISPR/Cas9-mediated gene targeting / 生理学报
Acta Physiologica Sinica
; (6): 482-490, 2021.
Article
em Zh
| WPRIM
| ID: wpr-887683
Biblioteca responsável:
WPRO
ABSTRACT
S100 calcium binding protein A9 (S100A9) is involved in a variety of biological processes such as inflammation and tumor cell migration and invasion regulation. The purpose of this study was to construct S100A9 gene-edited mice by using CRISPR/Cas9 technology, thereby providing an animal model for exploring the biological functions of this gene. According to the S100A9 gene sequence, the single-stranded small guide RNA (sgRNA) targeting exons 2 and 3 was transcribed in vitro, and a mixture of Cas9 mRNA and candidate sgRNA was injected into mouse fertilized eggs by microinjection. Early embryos were obtained and transferred to surrogate mice, and F
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Fenótipo
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Líquido da Lavagem Broncoalveolar
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Ovalbumina
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Camundongos Knockout
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Marcação de Genes
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Calgranulina B
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Modelos Animais de Doenças
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Técnicas de Inativação de Genes
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Sistemas CRISPR-Cas
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Pulmão
Limite:
Animals
Idioma:
Zh
Revista:
Acta Physiologica Sinica
Ano de publicação:
2021
Tipo de documento:
Article