Host Immune Responses to Intradiscal Gene Transfer / 대한척추외과학회지
Journal of Korean Society of Spine Surgery
; : 84-90, 2002.
Article
em Ko
| WPRIM
| ID: wpr-92548
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE: To elucidate host immune responses to intradiscal gene transfer. MATERIALS AND METHODS: Twenty rabbits were utilized. Ad/luciferase (adenovirus construct) were injected into nucleus pulposus of lumbar vertebrae. Group 1 received intradiscal injection of Ad/luciferase only, Group 2 received subcutaneous and intradiscal injection simultaneously, Group 3 received subcutaneous injection then intradiscal injection with 2 weeks interval. Blood samples were obtained serially after injection. Animals were sacrificed at 7 weeks. Antibody to adenovirus in peripheral blood was measured with ELISA and transgene expression was measured with standard luciferase kits. RESULTS: All rabbits in the Group 2 and 3 exhibited increased production of neutralizing antibody. There were clearly two subgroups in Group 1, three rabbits exhibited production of antibody but remaining three rabbits showed little or no production of antibody. All rabbits showed robust increase in transgene expression regardless of titer of neutralizing antibody. CONCLUSION: The intervertebral disc is favorable site for adenovirus-mediated gene transfer escaping from systemic immunity.
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Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Nações Unidas
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Ensaio de Imunoadsorção Enzimática
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Adenoviridae
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Transgenes
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Anticorpos Neutralizantes
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Injeções Subcutâneas
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Disco Intervertebral
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Luciferases
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Vértebras Lombares
Limite:
Animals
Idioma:
Ko
Revista:
Journal of Korean Society of Spine Surgery
Ano de publicação:
2002
Tipo de documento:
Article