(+)-corynoline Regulates the Proliferation,Stemness and Apoptosis of Triple Negative Breast Cancer Cells / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 244-252, 2022.
Article
em Zh
| WPRIM
| ID: wpr-927872
Biblioteca responsável:
WPRO
ABSTRACT
Objective To explore the performance and mechanism of(+)-corynoline in treating triple negative breast cancer MDA-MB-436 cells and thus provide an option for the development of drugs against this cancer. Methods The viability,proliferation,apoptosis and migration/invasion of MDA-MB-436 cells treated with(+)-corynoline were detected by CCK-8 assay,colony formation assay,flow cytometry and Transwell assay,respectively.Furthermore,Western blotting was employed to determine the expression of related proteins,and RNA-Seq was performed for the MDA-MB-436 cells treated with(+)-corynoline. Results (+)-corynoline inhibited the proliferation and stemness and promoted the apoptosis of MDA-MB-436 cells.Further,(+)-corynoline may activate the oxidative phosphorylation pathway to play a role in inhibiting triple negative breast cancer. Conclusion (+)-corynoline can inhibit triple negative breast cancer cells,which helps to address the poor efficacy of existing chemotherapeutics and facilitate the development of drugs against this cancer.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Alcaloides de Berberina
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Neoplasias da Mama
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Movimento Celular
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Apoptose
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Linhagem Celular Tumoral
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Proliferação de Células
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Neoplasias de Mama Triplo Negativas
Limite:
Female
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Humans
Idioma:
Zh
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2022
Tipo de documento:
Article