Application of single-cell transcriptome sequencing in mechanistic toxicology / 中华预防医学杂志
Chinese Journal of Preventive Medicine
; (12): 29-32, 2022.
Article
em Zh
| WPRIM
| ID: wpr-935246
Biblioteca responsável:
WPRO
ABSTRACT
Traditional bulk RNA sequencing assesses the average expression level of genes in tissues rather than the differences in cellular responses. Accordingly, it is hard to differentiate sensitive responding cells, leading to inaccurate identification of toxicity pathways. Single-cell RNA sequencing (scRNA-seq) isolated single cells from tissue and subjected them to cell subtypes-specific transcriptome analysis. This technique in toxicological studies realizes the heterogeneous cellular responses in the tissue microenvironment upon chemical exposure. Thus it helps to identify sensitive responding cells and key molecular events, providing a powerful tool and a new perspective for exploring the mechanisms of toxicity and the modes of action. This review summarizes the development, principle, method, application and limitations of scRNA-seq in mechanistic toxicological researches, and discusses the prospect of multi-directional applications.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Sequência de Bases
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Análise de Sequência de RNA
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Perfilação da Expressão Gênica
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Análise de Célula Única
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Transcriptoma
Idioma:
Zh
Revista:
Chinese Journal of Preventive Medicine
Ano de publicação:
2022
Tipo de documento:
Article