The in vivo imaging of zebrafish for chemical vascular toxicity assessment / 中华预防医学杂志
Chinese Journal of Preventive Medicine
; (12): 33-37, 2022.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-935247
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
To evaluate the vascular toxicity of chemicals by a real-time observation approach using the transgenic zebrafish.Methods:
The spatiotemporal vascular alterations of transgenic zebrafish after chemical exposure were assessed by laser confocal microscopy and high-content screening analysis, respectively.Results:
The method using Laser Confocal Microscopy (LCM) is easier to operate and yields high-resolution images, while it is lower throughput and inefficient. In contrast, high-content analysis (HCA) analysis obtains high-quality data of vascular toxicity manifesting whole blood vasculature, whereas it requires delicate operation procedures and advanced experimental conditions.Conclusion:
Two kinds of zebrafish imaging methods each have advantages and disadvantages. LCM is suitable for the evaluation of a small number of chemicals. HCA, a cutting-edge technology, has great potential for chemical safety assessment allowing high throughput vascular toxicity tests of a good number of chemicals at a time.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Peixe-Zebra
/
Animais Geneticamente Modificados
/
Sistema Cardiovascular
/
Testes de Toxicidade
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Preventive Medicine
Ano de publicação:
2022
Tipo de documento:
Artigo