Hens immunized with live attenuated Salmonella strains expressing virulence-associated genes in avian pathogenic Escherichia coli passively transfer maternal antibodies to chicks
Korean Journal of Veterinary Research
; : 167-176, 2016.
Article
em En
| WPRIM
| ID: wpr-94482
Biblioteca responsável:
WPRO
ABSTRACT
We investigated whether maternal antibodies (mAbs) elicited by dams immunized with recombinant vaccine candidates against avian pathogenic Escherichia coli (APEC) can passively confer protective immunity to chicks. In the present study, pBP244 plasmids carrying selected antigens of APEC were transformed into Salmonella Typhimurium JOL912, which was used as a vaccine candidate against APEC. The hens were immunized with the vaccine candidates using prime or booster doses. The levels of IgG and sIgA specific to the selected antigens increased significantly following prime immunization. To evaluate the persistence of passively transferred mAbs, the levels of IgY and IgA were determined in egg yolks and whites, respectively. The eggs from the immunized group showed consistently increased levels of IgY and IgA until week 16 post-laying (PL) and week 8 PL, respectively, relative to the control group. The presence of mAbs was observed in chicks that hatched from the hens, and titers of plasma IgY were consistently raised in those from the immunized hens by day 14 post-hatching. Further, chicks from the immunized hens were protected from challenge with a virulent APEC strain, whereas those from non-immunized hens showed acute mortality.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Óvulo
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Plasma
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Plasmídeos
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Salmonella
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Salmonella typhimurium
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Imunoglobulina A
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Imunoglobulina A Secretora
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Imunoglobulina G
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Mortalidade
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Imunização Passiva
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Korean Journal of Veterinary Research
Ano de publicação:
2016
Tipo de documento:
Article