Delayed allograft rejection by the suppression of class II transactivator
Experimental & Molecular Medicine
; : 210-216, 2006.
Article
em En
| WPRIM
| ID: wpr-96570
Biblioteca responsável:
WPRO
ABSTRACT
We examined the effect of class II transactivator (CIITA) down-modulation on allograft rejection. To inhibit the function of CIITA, we constructed a series of CIITA mutants and found one exhibiting the dominant-negative effect on the regulation of major histocompatibility complex (MHC) class II expression. To test whether the CIITA dominant-negative mutant reduces immunogenecity, CIITA-transfected melanoma cells were injected into allogeneic host and assessed for immune evading activity against host immune cells. We demonstrated that the CIITA dominant-negative mutant allowed tumor nodules to develop earlier in the lung than control by this tumor challenge study. Furthermore, skin grafts deficient for CIITA also survived longer than wild-type in allogeneic hosts. Both the tumor challenge and skin graft studies suggest the inhibition of CIITA molecules in donor tissue would be beneficial to the control of allo-response.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Transplante Homólogo
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Melanoma Experimental
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Camundongos Transgênicos
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Proteínas Nucleares
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Transfecção
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Antígenos de Histocompatibilidade Classe II
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Ativação Transcricional
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Transativadores
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Interferon gama
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Transplante de Pele
Limite:
Animals
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Humans
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Male
Idioma:
En
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2006
Tipo de documento:
Article