Chromosomal integration of large DNA fragments in microorganisms: a review / 生物工程学报
Chinese Journal of Biotechnology
; (12): 842-857, 2023.
Article
em Zh
| WPRIM
| ID: wpr-970409
Biblioteca responsável:
WPRO
ABSTRACT
The modern bio-fermentation industry requires design and creation of efficient microbial cell factories for directed conversion of raw materials to target products. The main criteria for assessing the performance of microbial cell factories are their product synthesis capacity and stability. Due to the deficiencies of plasmids in gene expression such as instability and being easy to lose, integration of genes into chromosome is often a better choice for stable expression in microbial hosts. To this end, chromosomal gene integration technology has received much attention and has developed rapidly. In this review, we summarize the recent research progresses of chromosomal integration of large DNA fragments in microorganisms, illustrate the principles and features of various technologies, highlight the opportunity brought by the CRISPR-associated transposon systems, and prospect future research direction of this technology.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Plasmídeos
/
DNA
/
Cromossomos
/
Clonagem Molecular
/
Fermentação
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2023
Tipo de documento:
Article