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Effect of sulfur fumigation on quality and safety of Lilii Bulbus / 中国中药杂志
Article em Zh | WPRIM | ID: wpr-970535
Biblioteca responsável: WPRO
ABSTRACT
Lilii Bulbus is a commonly used Chinese herbal medicine with both medicinal and edible values, while the market products usually has the problem of sulfur fumigation. Therefore, the quality and safety of Lilii Bulbus products deserve attention. In this study, ultra-high performance liquid chromatography-time of flight-tandem mass spectrometry(UPLC-Q-TOF-MS/MS) was combined with principal component analysis(PCA) and orthogonal partial least squares discriminant analysis(OPLS-DA) to analyze the differential components of Lilii Bulbus before and after sulfur fumigation. We identified ten markers generated after sulfur fumigation, summarized their mass fragmentation and transformation patterns, and verified the structures of phenylacrylic acid markers of sulfur fumigation. At the same time, the cytotoxicity of the aqueous extracts of Lilii Bulbus before and after sulfur fumigation was evaluated. The results showed that in the concentration range of 0-800 mg·L~(-1), the aqueous extract of Lilii Bulbus after sulfur fumigation had no significant effect on the viability of human liver LO2 cells, human renal proximal tubular HK-2 cells, and rat adrenal pheochromocytoma PC-12 cells. Moreover, the viability of the cells exposed to the aqueous extract of Lilii Bulbus before and after sulfur fumigation showed no significant difference. This study identified phenylacrylic acid and furostanol saponins as markers of sulfur-fumigated Lilii Bulbus for the first time, and made clear that proper sulfur fumigation of Lilii Bulbus would not produce cytotoxicity, providing a theoretical basis for the rapid identification and quality and safety control of sulfur-fumigated Lilii Bulbus.
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Texto completo: 1 Base de dados: WPRIM Assunto principal: Enxofre / Fumigação / Cromatografia Líquida de Alta Pressão / Células Epiteliais / Espectrometria de Massas em Tandem Limite: Animals / Humans Idioma: Zh Revista: China Journal of Chinese Materia Medica Ano de publicação: 2023 Tipo de documento: Article
Texto completo: 1 Base de dados: WPRIM Assunto principal: Enxofre / Fumigação / Cromatografia Líquida de Alta Pressão / Células Epiteliais / Espectrometria de Massas em Tandem Limite: Animals / Humans Idioma: Zh Revista: China Journal of Chinese Materia Medica Ano de publicação: 2023 Tipo de documento: Article
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