Hepatocyte nuclear factor 1-alpha mutation in normal glucose-tolerant subjects and early-onset type 2 diabetic patients
The Korean Journal of Internal Medicine
; : 165-169, 2008.
Article
em En
| WPRIM
| ID: wpr-97390
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND/AIMS:
The prevalence of diabetes in Korea is reported to be approximately 10%, but cases of maturity-onset diabetes of the young (MODY) are rare in Korea. A diagnostic technique for autosomal dominant MODY is being actively sought. In this regard, we used a DNA chip to investigate the frequency of mutations of the MODY3 gene (hepatocyte nuclear factor-1alpha) in Korean patients with early-onset type 2 diabetes.METHODS:
The genomic DNA of 30 normal individuals [age, 24.9+/-8.6 years] and 25 patients with early-onset type 2 diabetes (age, 27+/-5.9 years) was extracted, and the MODY3 gene was amplified. The amplified DNA was hybridized onto a MODY3 chip, which has oligonucleotides of 15-25 bases, representing wild-type and mutant MODY3 sequences in both forward and reverse orientations, immobilized on its surface.RESULTS:
Among the normal subjects, there was no mutation of MODY3. Among those with early-onset type 2 diabetes, there was one case of MODY3 mutation.CONCLUSIONS:
Our results indicate that MODY3 mutations are not rare in Korean early-onset type 2 diabetes patients in Korea and suggest that MODY3 mutations in patients with early-onset type 2 diabetes need to be further evaluated.Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Estudos de Casos e Controles
/
Prevalência
/
Estudos de Coortes
/
Idade de Início
/
Povo Asiático
/
Diabetes Mellitus Tipo 2
/
Fator 1-alfa Nuclear de Hepatócito
/
Coreia (Geográfico)
/
Mutação
Tipo de estudo:
Etiology_studies
/
Incidence_studies
/
Observational_studies
/
Prevalence_studies
/
Risk_factors_studies
Limite:
Adolescent
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Adult
/
Female
/
Humans
/
Male
País/Região como assunto:
Asia
Idioma:
En
Revista:
The Korean Journal of Internal Medicine
Ano de publicação:
2008
Tipo de documento:
Article