Mechanism of BCL2L2-PABPN1 expression induced by sodium arsenite and its metabolites in 16HBE cells / 中国职业医学
China Occupational Medicine
; (6): 522-2022.
Article
em Zh
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| ID: wpr-976131
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ABSTRACT
@#Objective - - (BCL2L2)- ( ) To investigate the differential expression of the fusion gene BCL 2 like protein 2 poly A (PABPN1) ( ) binding protein nuclear 1 induced by sodium arsenite SA and its methylated metabolites in 16HBE cells and the Methods ) , related mechanism. i The 16HBE cells exposed to SA at concentrations of 1.5 3.0 and 4.5 µmol/L were set as -, - - low medium and high dose arsenic exposure groups. The 16HBE cells exposed to 4.5 µmol/L monomethylarsonic acid ( ), ( ) , MMA dimethylarsonic acid DMA and SA were set as MMA group DMA group and SA group. The 16HBE cells without , BCL2L2-PABPN1 toxic stimulation were set as control group. After the cells were cultured for 48 hours the expression of was - ( - ) ) ( ) detected by quantitative real time polymerase chain reaction qRT PCR . ii Two small interfering RNA siRNA silencing 基金项目:国家自然科学基金( ); 年云南省科技厅昆明医科大学应用基础研究联合专项面上项目 82160607 2021 ( ) 202101AY070001-054 作者简介:施雅( —),女,在读大学本科生,主要从事劳动卫生与环境卫生学研究;尹锦瑶( —),女,在读劳动卫生与环境卫 2001 1995 生学硕士研究生,主要从事劳动卫生与环境卫生学研究;施雅和尹锦瑶为共同第一作者 通讯作者:何越峰教授,博士研究生导师,- : E mail heyuefeng@kmmu.edu.cn中国职业医学 年 月第 卷第 期 , , , · · 2022 10 49 5 Chin Occup Med October 2022 Vol.49 No.5 523 BCL2L2-PABPN1, - fragments were designed and transfected into 16HBE cells to knockdown which were set as siRNA 1 group - - BCL2L2-PABPN1 and siRNA 2 group. Non transfected control group without knockdown of transfection was set up. After , BCL2L2-PABPN1 - culturing for 48 hours the expression level of in the three groups of cells was detected by qRT PCR. The cell - survival rate and early apoptosis rate were detected by MTS method and JC 1 mitochondrial membrane potential detection , ( ) , method respectively. The apoptosis was detected by Hoechest33342/propidium iodide PI double staining and the expression - Results ) level of P53 signaling pathway related proteins was detected by Western blotting. i The relative expression of BCL2L2-PABPN1 (P ) BCL2L2- in 16HBE cells increased with the increasing SA doses <0.01 . The relative expression of PABPN1 - , - - in high dose arsenic exposure was higher than that in control group low dose and medium dose arsenic exposure ( P ) BCL2L2-PABPN1 , groups all <0.05 . The relative expression of in SA group was higher than those in control group MMA ( P ) BCL2L2-PABPN1 group and DMA group all <0.05 . The relative expression of showed no significant difference between , ( P ) ) BCL2L2-PABPN1 control group MMA group and DMA group all >0.05 . ii The relative expression levels of and cell - - - ( P ) survival rate in siRNA 1 group and siRNA 2 group were lower than those in non transfected control group all <0.05 . , (P ) However there was no significant difference in the early apoptosis rate among the three groups >0.05 . The results of - Hoechest33342/PI double staining showed that the number of nuclear shrinkage and early apoptotic cells in siRNA 1 group and - - , - siRNA 2 group was higher than that in non transfected control group. The relative protein expression levels of P53 phospho , - - , - - ( P ) p53 BCL 2 associated death promoter P21 and cytochrome C in siRNA 1 group and siRNA 2 group were higher all <0.05 , - - P and the relative protein expression levels of P53 up regulated modulator of apoptosis were lower (all <0.05), when compared - Conclusion with the non transfected control group. SA may block the apoptosis of 16HBE cells by inducing the expression of BCL2L2-PABPN1 fusion gene . The mechanism may be related to the activation of P53 signaling pathway. The SA methylated BCL2L2-PABPN1 BCL2L2-PABPN1 - metabolites MMD and DMA had no effect on the expression of . may affect anti apoptosis BCL2L2 PABPN1 through affecting the synergistic effect of and genes.
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Zh
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China Occupational Medicine
Ano de publicação:
2022
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Article