Vanilloid Receptor 1 Agonists, Capsaicin and Resiniferatoxin, Enhance MHC Class I-restricted Viral Antigen Presentation in Virus-infected Dendritic Cells
Immune Network
; : 233-241, 2016.
Article
em En
| WPRIM
| ID: wpr-97831
Biblioteca responsável:
WPRO
ABSTRACT
DCs, like the sensory neurons, express vanilloid receptor 1 (VR1). Here we demonstrate that the VR1 agonists, capsaicin (CP) and resiniferatoxin (RTX), enhance antiviral CTL responses by increasing MHC class I-restricted viral antigen presentation in dendritic cells (DCs). Bone marrow-derived DCs (BM-DCs) were infected with a recombinant vaccinia virus (VV) expressing OVA (VV-OVA), and then treated with CP or RTX. Both CP and RTX increased MHC class I-restricted presentation of virus-encoded endogenous OVA in BM-DCs. Oral administration of CP or RTX significantly increased MHC class I-restricted OVA presentation by splenic and lymph node DCs in VV-OVA-infected mice, as assessed by directly measuring OVA peptide SIINFEKL-Kb complexes on the cell surface and by performing functional assays using OVA-specific CD8 T cells. Accordingly, oral administration of CP or RTX elicited potent OVA-specific CTL activity in VV-OVA-infected mice. The results from this study demonstrate that VR1 agonists enhance anti-viral CTL responses, as well as a neuro-immune connection in anti-viral immune responses.
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Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Óvulo
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Células Receptoras Sensoriais
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Vaccinia virus
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Células Dendríticas
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Capsaicina
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Linfócitos T
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Administração Oral
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Apresentação de Antígeno
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Linfonodos
Limite:
Animals
Idioma:
En
Revista:
Immune Network
Ano de publicação:
2016
Tipo de documento:
Article