Oxidative stress induces autophagy to inhibit the proliferation and apoptosis of human bone marrow mesenchymal stem cells (hBMSCs) / 细胞与分子免疫学杂志
Chinese Journal of Cellular and Molecular Immunology
; (12): 626-632, 2023.
Article
em Zh
| WPRIM
| ID: wpr-981909
Biblioteca responsável:
WPRO
ABSTRACT
Objective To investigate the effect of H2O2-induced oxidative stress on autophagy and apoptosis of human bone marrow mesenchymal stem cells (hBMSCs). Methods hBMSCs were isolated and cultured. The cells were divided into control group, 3-MA group, H2O2 group, H2O2 combined with 3-MA group. DCFH-DA staining was used to analyze the level of reactive oxygen species (ROS). hBMSCs were treated with 0, 50, 100, 200, 400 μmol/L H2O2, and then the cell viability was detected by CCK-8 assay. The level of autophagy was detected by monodansylcadaverine (MDC) staining and LysoTracker Red staining. The cell apoptosis was detected by flow cytometry. Western blotting was used to detect the expression of beclin 1, mTOR, phosphorylated mTOR (p-mTOR), cleaved caspase-3(c-caspase-3) and caspase-3 proteins. Results Compared with the control group and 3-MA group, ROS level and autophagosomes were increased and the proliferation and apoptosis were decreased in H2O2 group. The protein expression of beclin 1, mTOR, c-caspase-3 was up-regulated, while the p-mTOR was down-regulated. Compared with the 3-MA group, the H2O2 combined with 3-MA group also had an increased ROS level and autophagosomes, but not with significantly increased apoptosis rate; The protein expression of beclin 1, mTOR, c-caspase-3 was up-regulated, and the p-mTOR was down-regulated. Conclusion H2O2 can induce hMSCs to trigger oxidative stress response. It enhances the autophagy and inhibits the proliferation and apoptosis of hBMSCs.
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Base de dados:
WPRIM
Assunto principal:
Autofagia
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Espécies Reativas de Oxigênio
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Apoptose
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Estresse Oxidativo
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Proliferação de Células
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Caspase 3
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Serina-Treonina Quinases TOR
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Células-Tronco Mesenquimais
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Proteína Beclina-1
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Peróxido de Hidrogênio
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Cellular and Molecular Immunology
Ano de publicação:
2023
Tipo de documento:
Article
País de publicação:
CHINA
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CN
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REPUBLIC OF CHINA