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The chemical reprogramming of unipotent adult germ cells towards authentic pluripotency and de novo establishment of imprinting
Protein & Cell ; (12): 477-496, 2023.
Article em En | WPRIM | ID: wpr-982528
Biblioteca responsável: WPRO
ABSTRACT
Although somatic cells can be reprogrammed to pluripotent stem cells (PSCs) with pure chemicals, authentic pluripotency of chemically induced pluripotent stem cells (CiPSCs) has never been achieved through tetraploid complementation assay. Spontaneous reprogramming of spermatogonial stem cells (SSCs) was another non-transgenic way to obtain PSCs, but this process lacks mechanistic explanation. Here, we reconstructed the trajectory of mouse SSC reprogramming and developed a five-chemical combination, boosting the reprogramming efficiency by nearly 80- to 100-folds. More importantly, chemical induced germline-derived PSCs (5C-gPSCs), but not gPSCs and chemical induced pluripotent stem cells, had authentic pluripotency, as determined by tetraploid complementation. Mechanistically, SSCs traversed through an inverted pathway of in vivo germ cell development, exhibiting the expression signatures and DNA methylation dynamics from spermatogonia to primordial germ cells and further to epiblasts. Besides, SSC-specific imprinting control regions switched from biallelic methylated states to monoallelic methylated states by imprinting demethylation and then re-methylation on one of the two alleles in 5C-gPSCs, which was apparently distinct with the imprinting reprogramming in vivo as DNA methylation simultaneously occurred on both alleles. Our work sheds light on the unique regulatory network underpinning SSC reprogramming, providing insights to understand generic mechanisms for cell-fate decision and epigenetic-related disorders in regenerative medicine.
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Texto completo: 1 Base de dados: WPRIM Assunto principal: Espermatogônias / Metilação de DNA / Células-Tronco Pluripotentes / Reprogramação Celular / Células-Tronco Pluripotentes Induzidas / Tetraploidia / Células Germinativas Limite: Animals Idioma: En Revista: Protein & Cell Ano de publicação: 2023 Tipo de documento: Article
Texto completo: 1 Base de dados: WPRIM Assunto principal: Espermatogônias / Metilação de DNA / Células-Tronco Pluripotentes / Reprogramação Celular / Células-Tronco Pluripotentes Induzidas / Tetraploidia / Células Germinativas Limite: Animals Idioma: En Revista: Protein & Cell Ano de publicação: 2023 Tipo de documento: Article