DNA analysis of a 500 year mummy sample / 法医学杂志

ABSTRACT

OBJECTIVE@#To accumulate experience for dated forensic matter analysis, for example, Mummy.@*METHODS@#DNA are extracted by methods of phenol-chloroform and are purified by Wizard DNA clean-up system. The STRs locus are ampolification with Promega Powerplus 16 system. The mtDNA hypervariable region 1 (HV1) is amplificated by '3 pair primers'. The products were sequenced with 377 DNA sequencer.@*RESULTS@#The STRs locus very distinctness and mtDNA sequence is correct.@*CONCLUSION@#It is a valuable method for special forensic matters.