Preliminary application on test paper of gold-labelled antibody against fibronectin EIIIA splicing variant / 法医学杂志
Journal of Forensic Medicine
; (6): 81-83, 2006.
Article
em Zh
| WPRIM
| ID: wpr-983142
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To develop the test Paper of gold-labelled antibody against fibronectin EIIIR A splicing variant, which can be available in forensic wound interval estimation.@*METHODS@#Two sensitive antibodies were compared with enzyme link immunoabsorband assay (ELISA). After colloid gold labeled, the effects of the two antibodies were tested by methods of Dot immunogold filtration assay and gold immunochromatography assay, respectively. The test paper was finally appraised by applied in experimental skin injury in rats.@*RESULTS@#On the test paper, detected line appeared in three hours wound age group, and then the darkness of positive staining became darker with injury time prolonging, while control normal skin cannot found to be positive staining.@*CONCLUSION@#The gold-labeled test paper can be useful in estimation of wounding interval in forensic science.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Fitas Reagentes
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Pele
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Fatores de Tempo
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Ferimentos não Penetrantes
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Ensaio de Imunoadsorção Enzimática
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Imunoensaio
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Distribuição Aleatória
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Fibronectinas
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Ratos Sprague-Dawley
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Processamento Alternativo
Limite:
Animals
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Ano de publicação:
2006
Tipo de documento:
Article