Molecular genetic analysis of Diego blood group Dia and Dib in Chinese Han population / 法医学杂志
Journal of Forensic Medicine
; (6): 283-285, 2007.
Article
em Zh
| WPRIM
| ID: wpr-983299
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To study the molecular genetic background of Diego blood group in Chinese Han population.@*METHODS@#A total of 2990 blood samples from unrelated blood donors were phenotyped for Dia and Dib by serological method. Twenty randomly selected samples of Di(a-b+) type and all of the samples of rare Di(a+b-) phenotype by screening were genotyped by PCR-SSP and direct DNA Sequencing.@*RESULTS@#Of the 2990 samples identified by serological method, 2821 were Di(a-b+), 167 were Di(a+b+) and 2 were Di(a+b-). All of the 20 randomly-selected samples with Di(a-b+) phenotype were DI2DI2 homozygote by PCR-SSP genotyping, with nucleotide C at nt position 2561 in exon 19 by direct sequencing of the DI gene. The 2 samples of rare Di (a+b-) phenotype were both the DI1DI1 homozygote, with nucleotide T at nt position 2561 in exon 19.@*CONCLUSION@#Our results indicate that the expression of Dia and Dib antigens in Chinese Han population most likely result from a single nucleotide T to C substitution at nucleotide position 2561 in exon 19 of the DI gene, which subsequently leads to an amino acid 854 change from Pro to Leu.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Fenótipo
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Doadores de Sangue
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Antígenos de Grupos Sanguíneos
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Tipagem e Reações Cruzadas Sanguíneas
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Dados de Sequência Molecular
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Sequência de Bases
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China
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Reação em Cadeia da Polimerase
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Éxons
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Análise de Sequência de DNA
Limite:
Humans
País/Região como assunto:
Asia
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Ano de publicação:
2007
Tipo de documento:
Article