Effects of serum from crush injury rats on vascular endothelial cell apoptosis and their potential mechanism / 法医学杂志
Journal of Forensic Medicine
; (6): 409-413, 2007.
Article
em Zh
| WPRIM
| ID: wpr-983327
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the effects of serum from crush injury rats on vascular endothelial cell apoptosis and their potential mechanism.@*METHODS@#Bovine aorta endothelial cells were cultured in vitro and the effects of serum from crush injury rats on cell apoptosis and intracellular calcium concentration ([Ca2+]i) were observed. Meanwhile, the levels of rat blood plasma endothelin-1 (ET-1) and atrial natriuretic peptide(ANP) were measured.@*RESULTS@#Compared with normal rat serum treatment, the cell apoptosis rate decreased from (8.26+/-1.75)% to (2.75+/-0.90)%, while the concentration of [Ca2+]i increased from (96.98+/-3.95) to (118.79+/-3.22) nmol/L in serum from crush injury rats, respectively. The concentration of ET-1 and ANP increased significantly in crush injury rat serum.@*CONCLUSION@#Serum from crush injury rats could inhibit apoptosis of the vascular endothelial cells. These effects may be related to increased level of [Ca2+]i mediated by ET-1 and ANP.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Células Cultivadas
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Cálcio
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Fator Natriurético Atrial
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Ratos Sprague-Dawley
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Apoptose
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Endotelina-1
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Meios de Cultura
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Células Endoteliais
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Modelos Animais de Doenças
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Extremidades
Limite:
Animals
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Ano de publicação:
2007
Tipo de documento:
Article