A new method for SNP typing based on allele specific PCR / 法医学杂志
Journal of Forensic Medicine
; (6): 189-193, 2008.
Article
em Zh
| WPRIM
| ID: wpr-983380
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To establish a new method of SNP typing.@*METHODS@#Based on the principle of allele specific PCR and capillary electrophoresis technique, 11 diallelic SNP loci were selected and two forward primers with different length were designed for each SNP, with their 3' ends matched to the two alleles, respectively. An artificially mismatched base was also introduced into the third or fourth base in the 3' end area of the two forward primers in order to enhance the specificity of amplification. A common reverse primer was designed 100-300 bp away from the forward primers, and labeled with fluorescence. The PCR products were separated and analyzed by ABI Prism 310 Genetic Analyzer after all of the 11 SNPs were multiply amplified.@*RESULTS@#A single product peak was observed while the SNP was homozygous, and two product peaks with different height were observed while the SNP was heterozygous. The length of PCR products was different with the different SNPs. According to the length of the products and the number of the product peaks, the genotypes of the 11 SNPs can simultaneously be analyzed, and the results were in accordance with the direct sequencing.@*CONCLUSION@#Fragment length discrepant allele specific fluorescence labeled multi-PCR (FLDASFLM-PCR) is a simple, rapid and efficient new method for SNP typing.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Reação em Cadeia da Polimerase
/
Eletroforese Capilar
/
Polimorfismo de Nucleotídeo Único
/
Alelos
/
Genética Forense
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Ano de publicação:
2008
Tipo de documento:
Article
País de publicação:
CHINA
/
CN
/
REPUBLIC OF CHINA