DNA degradation of porcine retinal cells for estimation of postmortem interval / 法医学杂志
Journal of Forensic Medicine
; (6): 250-252, 2010.
Article
em Zh
| WPRIM
| ID: wpr-983572
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To investigate DNA degradation of porcine retinal cells by single cell gel electrophoresis (SCGE) for estimation of postmortem interval (PMI).@*METHODS@#Degradation of retinal cells was observed by SCGE at 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 and 24h after death respectively, under the environmental conditions of being kept in dark place as well as a controlled temperature of (15 +/- 2) degrees C and humidity of (50 +/- 5)%. The comet pictures were captured by fluorescence microscope and analyzed by single cell gel electrophoresis (IMI 1.0).@*RESULTS@#From 2h to 24h postmortem, the degree of degradation of retinal DNA increased with the prolongation of PMI. The postmortem regression functions of head DNA%, L(T)/L(H), I(T)/I(H) were y = 92.227-5.188 x + 0.019 x2 + 0.001 x3 (R2 = 0.971), y = 0.035e(0.191x) (R2 = 0.947), y = 0.099e(0.264x) (R2 = 0.955), respectively.@*CONCLUSION@#The examination of retinal cell DNA degradation by SCGE is useful for estimating PMI.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Mudanças Depois da Morte
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Retina
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Suínos
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Fatores de Tempo
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Processamento de Imagem Assistida por Computador
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DNA
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Núcleo Celular
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Ensaio Cometa
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Patologia Legal
Limite:
Animals
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Ano de publicação:
2010
Tipo de documento:
Article