Determination of Sulfide Ion in Blood from Hydrogen Sulfide Poisoning Cases / 法医学杂志
Journal of Forensic Medicine
; (6): 148-153, 2017.
Article
em Zh
| WPRIM
| ID: wpr-984918
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVES@#To establish a gas chromatography-mass spectrometry (GC-MS) method for the determination of sulfide ion in blood and apply it to the practical cases.@*METHODS@#The 1, 3, 5-tribromobenzene was selected as an internal standard, and 0.2 mL blood sample was collected and analyzed using GC-MS after α-Bromo-2, 3, 4, 5, 6-pentafluorobenzyl bromide derivatization.@*RESULTS@#The mass concentration of sulfide ion in blood had good linearity in the range of 0.2-40 μg/mL with a limit of detection (LOD) of 0.05 μg/mL. The mass concentration of sulfide ion was less than 0.05 μg/mL in blank blood from different sources such as healthy subjects and dead cases. In 3 sulfide poisoning cases, sulfide ion was detected in the blood samples of 6 victims, and the mass concentration range was 1.02-3.13 μg/mL.@*CONCLUSIONS@#This study establishes a method for investigation of sulfide ion in blood which has been applied successfully to the cases of fatal sulfide poisonings.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Sulfetos
/
Limite de Detecção
/
Fluorbenzenos
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Sulfeto de Hidrogênio
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Cromatografia Gasosa-Espectrometria de Massas
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Ano de publicação:
2017
Tipo de documento:
Article