Development of a 2D-HPLC for simultaneous determination of rhynchophylline, isorhynchophylline, corynoxeine and isocorynoxeine / 四川精神卫生
Sichuan Mental Health
; (6): 424-428, 2022.
Article
em Zh
| WPRIM
| ID: wpr-987374
Biblioteca responsável:
WPRO
ABSTRACT
ObjectiveTo develop a two-dimensional high-performance liquid chromatography (2D-HPLC) for simultaneous determination of the contents of four kinds of Uncaria alkaloids: rhynchophylline, isorhynchophylline, corynoxeine and isocorynoxeine. MethodsThe 2D-HPLC apparatus was comprised of a first chromatographic column in version Aston SC2 (3.5 mm×25 mm, 5 μm), an intermediate column in version Aston SH C18 (3.5 mm×10 mm, 5 μm), and an analytical column in version Aston SCB (4.6 mm×125 mm, 5 μm). The mobile phase of the first and second liquid chromatography system were CAA-1 and mixed mobile phase (V BPI-1 basic mobile phase ∶ V MPI-1 mobile phase ∶ V OPI-1 organic mobile phase = 45∶14∶41). The chromatographic parameters included a flow rate of 1.0 mL/min, a column temperature of 40℃, a wavelength of 254 nm, an injection volume of 500 μL and a detection time of 9.5 min. ResultsThe linear ranges of rhynchophylline, isorhynchophylline, corynoxeine and isocorynoxeine were 9.77~10 000.00 ng/mL (r=0.999 6), 10.74~11 000.00 ng/mL (r=0.999 7), 10.74~11 000.00 ng/mL (r=0.999 7), 10.74~11 000.00 ng/mL(r=0.999 6), respectively. The relative standard deviation (RSD) of precision, stability and repeatability were all less than 5.00%. The accuracy was 95.20%~104.01%, and the recovery rate was 93.63%~101.38%. ConclusionThe 2D-HPLC developed for simultaneous determination of four kinds of alkaloids in Uncaria is simple and accurate, which can be used as a new method for quality control of Uncaria.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Sichuan Mental Health
Ano de publicação:
2022
Tipo de documento:
Article
País de publicação:
CHINA
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CN
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REPUBLIC OF CHINA