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Multidrug Resistance Mechanisms of Carbapenem Resistant Klebsiella pneumoniae Strains Isolated in Chongqing, China
Article em En | WPRIM | ID: wpr-99759
Biblioteca responsável: WPRO
ABSTRACT

BACKGROUND:

Carbapenem-resistant Klebsiella pneumoniae (CRKP) is considered a serious global threat. However, little is known regarding the multidrug resistance (MDR) mechanisms of CRKP. This study investigated the phenotypes and MDR mechanisms of CRKP and identified their clonal characteristics.

METHODS:

PCR and sequencing were utilized to identify antibiotic resistance determinants. Integron gene cassette arrays were determined by restriction fragment length polymorphism (RFLP) analysis. Multi-locus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) were used for epidemiological analysis. Plasmids were typed by using a PCR-based replicon typing and analyzed by conjugation and transformation assays.

RESULTS:

Seventy-eight strains were identified as resistant to at least one carbapenem; these CRKP strains had a high prevalence rate (38.5%, 30/78) of carbapenemase producers. Additionally, most isolates harbored MDR genes, including Extended spectrum β-lactamases (ESBLs), AmpC, and quinolone and aminoglycoside resistance genes. Loss of porin genes was observed, and Class 1 integron was detected in 66.7% of the investigated isolates. PFGE and MLST results excluded the occurrence of clonal dissemination among these isolates.

CONCLUSIONS:

A high prevalence of NDM-1 genes encoding carbapenem resistance determinants was demonstrated among the K. pneumoniae isolates. Importantly, this is the first report of bla(NDM-1) carriage in a K. pneumoniae ST1383 clone in China and of a MDR CRKP isolate co-harboring bla(NDM-1), bla(KPC-2), bla(CTX-M), bla(SHV), acc(6′)-Ib, rmtB, qnrB, and acc(6′)-Ib-cr.
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Texto completo: 1 Base de dados: WPRIM Assunto principal: Fenótipo / Plasmídeos / Pneumonia / Replicon / Polimorfismo de Fragmento de Restrição / Resistência Microbiana a Medicamentos / China / Reação em Cadeia da Polimerase / Prevalência / Eletroforese em Gel de Campo Pulsado Tipo de estudo: Prevalence_studies País/Região como assunto: Asia Idioma: En Revista: Annals of Laboratory Medicine Ano de publicação: 2017 Tipo de documento: Article País de publicação: COREA DEL SUR / COREIA DO SUL / KR / SOUTH KOREA
Texto completo: 1 Base de dados: WPRIM Assunto principal: Fenótipo / Plasmídeos / Pneumonia / Replicon / Polimorfismo de Fragmento de Restrição / Resistência Microbiana a Medicamentos / China / Reação em Cadeia da Polimerase / Prevalência / Eletroforese em Gel de Campo Pulsado Tipo de estudo: Prevalence_studies País/Região como assunto: Asia Idioma: En Revista: Annals of Laboratory Medicine Ano de publicação: 2017 Tipo de documento: Article País de publicação: COREA DEL SUR / COREIA DO SUL / KR / SOUTH KOREA