RESUMO
INTRODUCTION: Tuberculosis is a public health problem across Mexico. This paper aims to select a panel, with a minimum number of repetitive elements (MIRU-VNTR) for genotypic characterization of Mycobacterium tuberculosis (M. tuberculosis) clinical isolates. METHOD: In this study, a full panel of 24 MIRU-VNTR loci was used to discriminate 65 clinical isolates of M. tuberculosis from three different geographical regions of Mexico. Those loci with the highest discriminatory power were subsequently selected. RESULTS: The panel, including five loci, was obtained by selecting the highest values of allelic diversity among the genotypes obtained. The dendrogram, generated by the panel MIRU-VNTR 5, showed a high discriminatory power with 65 unique genotype profiles and formed clusters according to the geographical region of origin. CONCLUSIONS: The panel MIRU-VNTR 5 can be useful for characterizing clinical isolates of M. tuberculosis in Mexico.
Assuntos
DNA Bacteriano/genética , Genes Bacterianos , Técnicas de Genotipagem , Sequências Repetitivas Dispersas , Programas de Rastreamento/métodos , Repetições Minissatélites , Mycobacterium tuberculosis/classificação , Tuberculose/microbiologia , Técnicas de Tipagem Bacteriana , Variação Genética , Genótipo , Geografia Médica , Humanos , México/epidemiologia , Mycobacterium tuberculosis/genética , Mycobacterium tuberculosis/isolamento & purificação , Filogenia , Tuberculose/epidemiologiaRESUMO
Mycobacterium bovis is the major causative agent of bovine tuberculosis, one of the most relevant zoonoses in the world, and affects a wide range of wild and domesticated animals. Development of screening panels in mycobacterial genotyping, according to specific geographical regions, is strongly needed. The aim of this study is to select a panel, constituted by highly polymorphic MIRU-VNTR loci, to discriminate clinical isolates of M. bovis in Mexico. In this study, 65 isolates of M. bovis obtained from clinical bovine samples proceeding from different geographic regions of Mexico were identified by phenotypic and genotypic tests and subsequently genotyped by a 24-locus MIRU-VNTR panel. The most polymorphic loci were selected to build a panel with a high discriminatory power similar to the 24-locus panel results. A panel of seven elements (QUB 11a, MIRU 26, ETR-A, QUB 26, MIRU 16, MIRU 27, and MIRU 39) with the highest allelic diversity showed an appropriate differentiation. The selected MIRU-VNTR elements, according to the regional allelic variability, may be used in the preliminary genotyping of Mycobacterium bovis isolates in Mexico.
Assuntos
Genótipo , Repetições Minissatélites/genética , Mycobacterium bovis/genética , Tuberculose Bovina/genética , Animais , Técnicas de Tipagem Bacteriana , Bovinos , Variação Genética , Mycobacterium bovis/patogenicidade , Tuberculose Bovina/microbiologiaRESUMO
INTRODUCCIÓN: La tuberculosis es un problema de salud pública que se distribuye de forma diferenciada en regiones de México. El presente trabajo propone un panel, con el mínimo número de MIRU-VNTR (mycobacterial interspersed repetitive unit-variable number tandem repeats), para la diferenciación preliminar de aislamientos clínicos de Mycobacterium tuberculosis (M. tuberculosis). MÉTODO: Se realizó el panel completo de 24 MIRU-VNTR a 65 aislamientos clínicos de M. tuberculosis provenientes de diferentes regiones geográficas de México. RESULTADOS: Se presenta un panel de cinco loci MIRU-VNTR para discriminar aislamientos clínicos de M. tuberculosis de tres diferentes regiones geográficas de México. CONCLUSIONES: La utilización del panel MIRU-VNTR 5 podría utilizarse como tamizaje durante la caracterización genotípica de aislamientos clínicos de M. tuberculosis en México
INTRODUCTION: Tuberculosis is a public health problem across Mexico. This paper aims to select a panel, with a minimum number of repetitive elements (MIRU-VNTR) for genotypic characterization of Mycobacterium tuberculosis (M. tuberculosis) clinical isolates. METHOD: In this study, a full panel of 24 MIRU-VNTR loci was used to discriminate 65 clinical isolates of M. tuberculosis from three different geographical regions of Mexico. Those loci with the highest discriminatory power were subsequently selected. RESULTS: The panel, including five loci, was obtained by selecting the highest values of allelic diversity among the genotypes obtained. The dendrogram, generated by the panel MIRU-VNTR 5, showed a high discriminatory power with 65 unique genotype profiles and formed clusters according to the geographical region of origin. CONCLUSIONS: The panel MIRU-VNTR 5 can be useful for characterizing clinical isolates of M. tuberculosis in Mexico