[Comparative characteristics of transcription of genes for alpha and beta-lymphotoxin subunits in B- and T-lymphocyte lines, in peripheral lymphocytes, and in normal human tissue]. / Sravnitel'naia kharakteristika transkriptsii genov sub''edinits alpha i beta limfotoksina v liniiakh B- i T-limfotsitov, v perifericheskikh limfotsitakh i v normal'nykh tkaniakh cheloveka.

Membrane lymphotoxin (LT) is heterotrimer LT alpha 1 beta 2, and its production depends on two genes. Northern blotting was employed in studying their transcription in B- and T-lymphoma cell lines and in peripheral blood lymphocytes before and after induction with phorbol myristate acetate (PMA). Transcription of either gene proved similarly regulated in several cell lines and in blood lymphocytes. Activation of the LT alpha gene was associated with induction of transcription factor NF-kappa B (p50/p65) upon cell treatment with PMA. On evidence of RT-PCR, two transcripts of the LT beta gene occurred in equimolar amounts in all lymphoid cells. A product of alternative splicing contained an open reading frame coding for the cytoplasmic portion of LT beta.

[Heterologous expression of murine lymphotoxins in Escherichia coli and preparation of antibodies]. / Geterologicheskaia ékspressiia limfotoksinov myshi v kletkakh Escherichia coli i poluchenie antitel k nim.

Genes encoding fragments of polypeptide chains of murine lymphotoxins (LT), namely, LT-alpha truncated from the N-terminus and the LT-beta extracellular domain, containing N-terminal hepta- and hexahistidine epitopes, respectively, were expressed in E. coli cells. The recombinant proteins purified by metallochelate chromatography were used to obtain polyclonal antibodies that specifically recognize murine LT.

[Bacterial synthesis of immunogenic epitopes of foot-and-mouth disease virus fused either to human necrosis factor or to hepatitis B core antigen]. / Bakterial'nyi sintez immunogennykh épitopov virusa iashchura v sostave gibridov s faktorom nekroza opukholei i kor-antigenom virusa gepatita B.

Using recombinant DNA technology, construction and bacterial expression of genes was carried out which code for hybrid proteins, human tumor necrosis factor and hepatitis B core protein fused to immunogenic epitopes of foot-and-mouth disease virus, strains A22 and O1-194. Hybrids of tumor necrosis factor with foot-and-mouth disease antigenic determinants protected laboratory animals against the experimental challenge with a homologous strain of foot-and-mouth disease virus. Hybrid protein that contained immunogenic regions of two strains, A22 and O1-194, protected animals against infection with both A and O serotypes. Hybrid proteins based on hepatitis B virus core antigen retained the ability to assemble into core-like particles.