RESUMEN
The global structure of microwave-assisted flash-extracted pectins isolated from fresh sugar beet pulp has been studied. The objective was to minimize the disassembly and possibly the degradation of pectin molecules during extraction. These pectins have been characterized by high-performance size exclusion chromatography with light scattering, viscometric detection, and atomic force microscopy (AFM). Analysis of molecular parameters was performed on 15 and 8 microm size column packings. Samples analyzed with 15 microm packing gave weight-average molar masses that ranged from 532,000 to 1.2 million Da, radii of gyration from about 35 to 51 nm, polydispersities from 1.78 to 2.58, intrinsic viscosities from about 3.00 to 4.30 dL/g, and recoveries from 8.40 to 14.81% of dry weight. Chromatography revealed that a bimodal distribution of high molar mass spherical particles and lower molar mass coils was obtained. AFM images of pectin corroborated this conclusion and further revealed that these strands and spherical particles were integrated into networks. It is demonstrated that microwave-assisted extraction of sugar beet pulp under moderate pressure and at relatively low temperature could extract under acid conditions high molar mass, moderate-viscosity pectin in minutes rather than hours as required by conventional heating.
Asunto(s)
Beta vulgaris/química , Microondas , Pectinas/química , Pectinas/aislamiento & purificación , Dispersión de Radiación , Cromatografía Líquida de Alta Presión/métodos , Microscopía de Fuerza Atómica/métodos , Tamaño de la Partícula , Presión , ViscosidadRESUMEN
Composite films were prepared from pectin and fish skin gelatin (FSG) or pectin and soybean flour protein (SFP). The inclusion of protein promoted molecular interactions, resulting in a well-organized homogeneous structure, as revealed by scanning electron microscopy and fracture-acoustic emission analysis. The resultant composite films showed an increase in stiffness and strength and a decrease in water solubility and water vapor transmission rate, in comparison with films cast from pectin alone. The composite films inherited the elastic nature of proteins, thus being more flexible than the pure pectin films. Treating the composite films with glutaraldehyde/methanol induced chemical cross-linking with the proteins and reduced the interstitial spaces among the macromolecules and, consequently, improved their mechanical properties and water resistance. Treating the protein-free pectin films with glutaraldehyde/methanol also improved the Young's modulus and tensile strength, but showed little effect on the water resistance, because the treatment caused only dehydration of the pectin films and the dehydration is reversible. The composite films were biodegradable and possessed moderate mechanical properties and a low water vapor transmission rate. Therefore, the films are considered to have potential applications as packaging or coating materials for food or drug industries.
Asunto(s)
Peces , Gelatina/química , Pectinas/química , Piel/química , Proteínas de Soja/química , Animales , Fenómenos Químicos , Química Física , Embalaje de Medicamentos/instrumentación , Embalaje de Alimentos/instrumentación , Mecánica , Microscopía Electrónica de RastreoRESUMEN
Novel complex hydrogel beads were prepared from two edible polymers: pectin, a carbohydrate from citrus fruits, and zein, a protein from corn. The pectin/zein complex hydrogels did not swell in physiological environments, but hydrolyzed in the presence of pectinases. An in vitro study showed the capacity of the hydrogels to endure protease attack and residence time variation. The physical and biological properties of the new hydrogels were attributed to molecular entanglement of the two polymers. The pectin networks were stabilized by the bound zein molecules. In turn, the pectin networks shielded the bound zein from protease digestion.
Asunto(s)
Colon/fisiología , Sistemas de Liberación de Medicamentos , Excipientes , Pectinas/química , Zeína/química , Antiinflamatorios no Esteroideos/administración & dosificación , Cloruro de Calcio/química , Preparaciones de Acción Retardada , Dextranos/administración & dosificación , Composición de Medicamentos , Hidrogeles , Concentración de Iones de Hidrógeno , Indometacina/administración & dosificación , Microscopía Electrónica de Rastreo , Microscopía Fluorescente , Microesferas , Péptido Hidrolasas/química , Albúmina Sérica Bovina/administración & dosificaciónRESUMEN
Pectins of low and high degrees of esterification, as well as pectin derivatives carrying primary amines, were investigate for gel forming ability with mucosal tissues. The combination of scanning electronic microscopy and small deformation dynamic mechanical studies revealed that pectins with higher net electrical charges are more bioadhesive than the less charged ones. Both the negatively charged pectin formulation, P-25, and the positively charged formulation, P-N, were able to synergize with the mucus to produce rheologically strengthened gels. The highly esterified pectin, P-94, also synergized with the mucosal glycoproteins to form a gel structure via coil entanglements. The ex vivo studies further confirmed the microstructures of mucus gel networks with adsorbed pectins. When incubated with porcine intestinal mucus membrane, P-94 gels were found generally bound to the lumen area, P-25 gels were able to penetrate deeply near the wall area, P-N gels interacted with mucins via electrostatic bonding and dispersed into the whole area from the lumen to the wall. Hence, both P-N and P-94, by enhancing the protective barrier properties of mucus systems, may be useful alternatives for the treatment of mucus related irritation and infection. In drug-delivery systems, P-N and P-25 would deliver incorporated drugs mainly by pectin dissolution, while a diffusion mechanism would release drugs from P-94 gels.
Asunto(s)
Materiales Biocompatibles/química , Colon/metabolismo , Pectinas/química , Animales , Colon/efectos de los fármacos , Difusión , Sistemas de Liberación de Medicamentos , Geles , Concentración de Iones de Hidrógeno , Microscopía Confocal , Microscopía Electrónica de Rastreo , Mucinas/química , Pectinas/farmacología , Estructura Terciaria de Proteína , Reología , Porcinos , Termodinámica , Factores de TiempoRESUMEN
Sugar beet pulp and poly(lactic acid) (PLA) composites were prepared by compression-heating. The resultant thermoplastics had a lower density, but they had tensile strength similar to that of pure PLA specimens as well as the same geometric properties. Tensile properties depended on the initial water content of sugar beet pulp and the process by which composites were manufactured. In comparison with sugar beet pulp, the composite showed improved water resistance. This can be attributed to the hydrophobic character of PLA and pulp-matrix interactions. The composite thermoplastics showed suitable properties for potential use as lightweight construction materials.
Asunto(s)
Beta vulgaris/química , Ácido Láctico/química , Polímeros/química , Fenómenos Químicos , Química Física , Interacciones Hidrofóbicas e Hidrofílicas , Microscopía Electrónica de Rastreo , Poliésteres , Resistencia a la Tracción , Agua/análisisRESUMEN
The thermally tolerant pectin methylesterase (TT-PME) was isolated as a monocomponent enzyme from sweet orange fruit (Citrus sinensis var. Valencia). It was also isolated from flower and vegetative tissue. The apparent molecular weight of fruit TT-PME was 40800 by SDS-PAGE and the isoelectric point estimated as pI 9.31 by IEF-PAGE. MALDI-TOF MS identified no tryptic-peptide ions from TT-PME characteristic of previously described citrus PMEs. TT-PME did not absolutely require supplemented salt for activity, but salt activation and pH-dependent activity patterns were intermediate to those of thermolabile PMEs. Treatment of non-calcium-sensitive pectin with TT-PME (reducing the degree of methylesterification by 6%) increased the calcium-sensitive pectin ratio from 0.01 to 0.90, indicating a blockwise mode of action. TT-PME produced a significantly lower end-point degree of methylesterification at pH 7.5 than at pH 4.5. Extensive de-esterification with TT-PME did not reduce the pectin molecular weight or z-average radius of gyration, as determined by HPSEC.
Asunto(s)
Hidrolasas de Éster Carboxílico/aislamiento & purificación , Hidrolasas de Éster Carboxílico/metabolismo , Citrus sinensis/enzimología , Calor , Pectinas/metabolismo , Frutas/enzimología , Hojas de la Planta/químicaRESUMEN
Using citronellal as a model compound, pectin gels formulations were evaluated for the controlled fragrance release by kinetic and static methods. The pectins with higher degrees of esterification induced a stronger molecular association with the nonpolar fragrance. This resulted in a prolonged duration of fragrance release and the limitation of fragrance adsorption to the receptor skin layers. The increase in pectin concentrations suppressed the fragrance release by a diffusion mechanism. Blocking the carboxyl groups of pectin with calcium ions reduces the hydrophilicity of pectin and provides physical barriers for citronellal diffusion. The pectin/calcium microparticles are promising materials for controlled fragrance release.
Asunto(s)
Aldehídos/farmacocinética , Preparaciones de Acción Retardada/farmacocinética , Monoterpenos/farmacocinética , Pectinas/química , Perfumes/farmacocinética , Monoterpenos Acíclicos , Adsorción , Aldehídos/química , Preparaciones de Acción Retardada/química , Difusión , Esterificación , Geles , Monoterpenos/química , Perfumes/química , Vehículos Farmacéuticos , Tecnología Farmacéutica/métodos , Viscosidad , VolatilizaciónRESUMEN
Pectin-derived matrices are now being examined and tested for controlled drug delivery. Pectin is intact in the upper gastrointestinal tract and degraded by colonic microflora. The composition of this microflora remains relatively consistent across a diverse human population. Thus, pectin-derived drug carriers provide promising potential for colon-specific drug delivery. This paper reviews recent developments in pectin-derived formulations. Subjects reviewed include gelation of pectin, calcium cross-linked pectinate, composites of pectin and other polymers, technologies to fabricate pectin into useful drug delivery vehicles, and methods to evaluate release kinetics of incorporated drugs. This article discusses advantages, limitations, and possible future developments in pectin-based formulations with particular emphasis on the field of colon-specific drug delivery.
Asunto(s)
Enfermedades del Colon/terapia , Sistemas de Liberación de Medicamentos , Pectinas/química , Administración Oral , Animales , Calcio/química , Cationes , Celulosa/química , Colon/metabolismo , Relación Dosis-Respuesta a Droga , Portadores de Fármacos , Humanos , Modelos Químicos , Polímeros/químicaRESUMEN
Pectin gels were induced by monovalent salts (0.2 M) concurrently with deesterification of high methoxy pectin using a salt-independent orange pectin methylesterase (PME). Constant pH was maintained during deesterification and gelation. If salt or PME was absent, the pectin did not form a gel. The gel strength was influenced by both pH and species of monovalent cation. At pH 5.0, the pectin gel induced by KCl was significantly stronger than the NaCl-induced gel. In contrast, a much stronger gel was produced in the presence of NaCl as compared to KCl at pH 7.0. LiCl did not induce pectin gelation at either pH. Molecular weights of pectins increased from 1.38 x 10(5) to 2.26 x 10(5) during NaCl-induced gelation at pH 7. One proposal to explain these pectin molecular weight changes is a hypothetical PME transacylation mechanism. However, these pectin molecular weight changes can also be explained by metastable aggregation of the enzymatically deesterified low methoxy pectin. We postulate that gelation was induced by a slow deesterification of pectin under conditions that would normally salt out (precipitate) low methoxy pectin in the absence of PME.
Asunto(s)
Pectinas/química , Pectinas/metabolismo , Sales (Química)/farmacología , Proteínas Virales/metabolismo , Geles/química , Concentración de Iones de Hidrógeno , Cloruro de Litio/farmacología , Peso Molecular , Proteínas de Movimiento Viral en Plantas , Cloruro de Potasio/farmacología , Cloruro de Sodio/farmacologíaRESUMEN
A commercial high-methoxy citrus pectin was treated with a purified salt-independent pectin methylesterase (PME) isozyme isolated from Valencia orange peel to prepare a series of deesterified pectins. A series of alkali-deesterified pectins was also prepared at pH 10 under conditions permitting beta-elimination. Analysis of these pectins using high-performance size exclusion chromatography (HPSEC) with on-line multiangle laser light-scattering, differential viscometer, and refractive index (RI) detectors revealed no reduction in weight-average molecular weight (M(w); 150000) in the PME-treated pectin series, whereas a 16% reduction in intrinsic viscosity (IV) occurred below a degree of esterification (DE) of 47%. In contrast, alkali deesterification rapidly reduced both M(w) and IV to less than half of that observed for untreated pectin. PME treatment of a non-calcium-sensitive citrus pectin introduced calcium sensitivity with only a 6% reduction in the DE. Triad blocks of unesterified galacturonic acid were observed in (1)H nuclear magnetic resonance spectra of this calcium-sensitive pectin (CSP). These results demonstrate that the orange salt-independent PME isozyme utilizes a blockwise mode of action. This is the first report of the preparation of a CSP by PME treatment without significant loss of the pectin's M(w) due to depolymerization.
Asunto(s)
Calcio/farmacología , Pectinas/química , Pectinas/metabolismo , Cromatografía Líquida de Alta Presión , Citrus/química , Citrus/enzimología , Esterificación , Concentración de Iones de Hidrógeno , Isoenzimas/metabolismo , Espectroscopía de Resonancia Magnética , Peso Molecular , Proteínas de Movimiento Viral en Plantas , Proteínas Virales/metabolismo , ViscosidadRESUMEN
A pectin methylesterase (PME) from sweet orange fruit rag tissue, which does not destabilize citrus juice cloud, has been characterized. It is a salt-dependent PME (type II) and exhibits optimal activity between 0.1 and 0.2 M NaCl at pH 7.5. The pH optimum shifted to a more alkaline range as the salt molarity decreased (pH 8.5-9.5 at 50 mM NaCl). It has an apparent molecular mass of 32.4 kDa as determined by gel filtration chromatography, an apparent molecular mass of 33.5 kDa as determined by denaturing electrophoresis, and a pI of 10.1 and exhibits a single activity band after isoelectric focusing (IEF). It has a K(m) of 0.0487 mg/mL and a V(max) of 4.2378 nkat/mg of protein on 59% DE citrus pectin. Deblocking the N-terminus revealed a partial peptide composed of SVTPNV. De-esterification of non-calcium-sensitive pectin by 6.5% increased the calcium-sensitive pectin ratio (CSPR) from 0.045 +/- 0.011 to 0.829 +/- 0.033 but had little, if any, effect on pectin molecular weight. These properties indicate this enzyme will be useful for studying the PME mode of action as it relates to juice cloud destabilization.
Asunto(s)
Hidrolasas de Éster Carboxílico/aislamiento & purificación , Hidrolasas de Éster Carboxílico/metabolismo , Citrus sinensis/enzimología , Frutas/enzimología , Cloruro de Sodio/farmacología , Cromatografía en Gel , Electroforesis en Gel de Poliacrilamida , Concentración de Iones de Hidrógeno , Focalización Isoeléctrica , Punto Isoeléctrico , Isoenzimas/aislamiento & purificación , Isoenzimas/metabolismo , Peso Molecular , Pectinas/metabolismoRESUMEN
Molecular interactions between ß-lactoglobulin (ß-LG) and sugar beet pectin (SBP) were studied using online multi-detection high performance size exclusion chromatography (HPSEC) at neutral pH and 50mM ionic strength. The hydrodynamic properties of various interacting polymer fractions were characterized in detail and compared with those of ß-LG and SBP. Results showed that â¼6.5% (w/w) of native dimeric ß-LG molecules formed complexes with over 35% SBP molecules of varying sizes, 800, 110 and 75 kDa. Although the ß-LG molecules bind to SBP molecules of all sizes and shapes, they tend to favor the intermediate (110 kDa) and small sized (75 kDa) SBP molecules. All resulting complexes possess altered shapes and hydrodynamic properties when compared to unbound SBP and ß-LG. About half of the interacting ß-LG (â¼3.5%) molecules were thought to bind to a small amount of non-covalently bound feruloyl groups, possibly present in SBP. When pre-heat treated ß-LG and SBP were combined, more than 16% of ß-LG formed complexes with at least 45% of SBP molecules of varying sizes, Mwâ¼750-800, 110, and 55-80 kDa. The complexes formed between ß-LG aggregates and/or oligomers and the large SBP molecules (750-800 kDa) adopt the shape of ß-LG aggregates, random coil. Both groups of complexes formed between ß-LG intermediate (110 kDa) and small sized (55-80 kDa) SBP take on the shape of rigid rod. It was speculated that half of the interacting heat-treated ß-LG molecules (â¼8%) are complexed with non-covalently bound feruloyl groups in SBP.
Asunto(s)
Beta vulgaris/química , Cromatografía en Gel , Lactoglobulinas/metabolismo , Pectinas/metabolismo , Calor , Hidrodinámica , Concentración de Iones de Hidrógeno , Lactoglobulinas/química , Peso Molecular , Concentración Osmolar , Pectinas/química , Unión ProteicaRESUMEN
We have solubilized and separated polysaccharides from sugar beet pulp (SBP) into three fractions with steam assisted flash extraction (SAFE). For pectin, recovery ranged from 8 to 14%, degree of methy-esterification 66-73%, crude protein 1.3-1.7%, M(w) 262-318 kDa, η(w) 0.22-0.23 dL/g, Rg(z) 36-39 nm and Rh(z) 41-42 nm. For alkaline soluble polysaccharides, (ASP I) recovery ranged from 4.0 to 6.5%, crude protein 1.2-4.8%, weight average molar mass (M(w)) 66-68 kDa, weight average intrinsic viscosity (η(w)) 0.27-0.30 dL/g, z-average radius of gyration (Rg(z)) 25-29 nm and z-average hydrated radius (Rh(z)) 10-11 nm. ASP II recovery ranged from 2.0 to 8.6%, crude protein 1.2-4.8%, M(w) 299-339 kDa, η(w) 0.22-0.33 dL/g, Rg(z) 33-34 nm and Rh(z) 30-34 nm. Recovery of the residue mainly cellulose, ranged from 20.3 to 22.3%. The cellulose in this fraction was converted to carboxymethyl cellulose (CMC). The CMC fraction contained 0.33-0.43 crude protein and had an M(w) ranging from 127 to 263 kDa, η(w) 3.6-8.0 dL/g, Rg(z) 35-45 nm and Rh(z) 27-40 nm.
Asunto(s)
Beta vulgaris/química , Fenómenos Químicos , Proteínas de Plantas/química , Polisacáridos/química , Polisacáridos/aislamiento & purificación , Carboximetilcelulosa de Sodio/química , Pectinas/química , SolubilidadRESUMEN
UNLABELLED: To improve extraction yield of pumpkin pectin, microwave heating was adopted in this study. Using hot acid extraction, pumpkin pectin yield decreased from 5.7% to 1.0% as pH increased from pH 1.0 to 2.0. At pH 2.5, no pectin was recovered from pumpkin flesh powder. After a pretreatment at pH 1.0 and 25 °C for 1 h, pumpkin powder was microwave-extracted at 120 °C for 3 min resulting in 10.5% of pectin yield. However, premicrowave treatment at 60 °C for 20 min did not improve extraction yield. When microwave heating at 80 °C for 10 min was applied after premicrowave treatment, final pectin yield increased to 11.3%. When pH was adjusted to 2.0, the yield dropped to 7.7% under the same extraction conditions. Molecular shape and properties as well as chemical composition of pumpkin pectin were significantly affected depending on extraction methods. Galacturonic acid content (51% to 58%) of pumpkin pectin was lower than that detected in commercial acid-extracted citrus pectin, while higher content of neutral sugars and acetyl esters existed in pumpkin pectin structure. Molecular weight (M(w) ) and intrinsic viscosity (η(w) ) determined for microwave-extracted pumpkin pectins were substantially lower than acid-extracted pectin, whereas polydispersity was greater. However, microwave-extracted pectin at pH 2.0 had more than 5 times greater M(w) than did the pectin extracted at pH 1.0. The η(w) of microwave-extracted pectin produced at pH 2.0 was almost twice that of other microwave-extracted pectins, which were comparable to that of acid-extracted pectin. These results indicate that extraction yield of pumpkin pectin would be improved by microwave extraction and different pectin structure and properties can be obtained compared to acid extraction. PRACTICAL APPLICATION: Pumpkin is a promising alternative source for pectin material. Pumpkin pectin has a unique chemical structure and physical properties, presumably providing different functional properties compared to conventional commercial pectin sources. Depending on the conditions to produce pumpkin pectin, diverse molecular structures can be obtained and utilized in various food applications.
Asunto(s)
Cucurbita/química , Manipulación de Alimentos/métodos , Microondas , Pectinas/química , Pectinas/aislamiento & purificación , Carbohidratos/análisis , Cromatografía de Gases y Espectrometría de Masas , Ácidos Hexurónicos/análisis , Calor , Concentración de Iones de Hidrógeno , Estructura Molecular , Peso Molecular , ViscosidadRESUMEN
Images of native high-methoxyl sugar acid gels (HMSAGs) were obtained by atomic force microscopy (AFM) in the Tapping Mode. Electronic thinning of the pectin strands to one-pixel wide allowed the pectin network to be viewed in the absence of variable strand widths related to preferentially solvated sugar. Thinned images revealed that HMSAGs of pectin comprise a partially cross-linked network, in that many of the cross-linking moieties are attached at only one end. Based on their structural similarities, aggregated pectin in water appears to be a fluid precursor of a HMSAG of pectin. Furthermore, examination of AFM images revealed that gels with 'uniform' distribution of strands and pores between strands had higher gel strengths than gels in which strands were non-uniformly distributed and were separated by large and small spaces.
Asunto(s)
Pectinas/química , Citrus/química , Geles/química , Microscopía de Fuerza Atómica/métodos , Estructura Molecular , Pectinas/ultraestructura , Azúcares Ácidos/químicaRESUMEN
Several studies have suggested that the emulsification properties associated with pectin obtained from sugar beet (Beta vulgaris) are due to the presence of a protein-pectin complex. Nevertheless, the identity of the protein has remained elusive. Pectin, extracted from sugar beet pulp by microwave-assisted extraction, and a commercial sample were both subjected to protease digestion with trypsin. The resulting peptides were separated from the pectin solution by ultrafiltration using a 3 kDa molecular weight cutoff (MWCO) membrane and analyzed using matrix-assisted laser desorption ionization with tandem time-of-flight mass spectrometry. The partial sequences derived from the mass spectrometry analyses of the resulting tryptic peptides are found to be highly consistent with extensin protein matched from the B. vulgaris Genetic Index database and also correspond to previously reported extensin peptides found in sugar beet cell suspension cultures. Further attempts were made to disassociate the protein from pectin using 1 M NaCl and a 100 kDa MWCO membrane; however, no peptides were observed following trypsin digestion of the permeate solution. This evidence suggests the existence of a complex between the pectin and extensin that is not due to ionic interactions. Trypsin digestion of commercial sugar beet pectin also produced the peptide profile observed with the microwave-assisted extracted pectin sample. Atomic force microscopy established that the number of rod-like elements decreased following protease treatment compared to the untreated sample.
Asunto(s)
Beta vulgaris/química , Glicoproteínas/análisis , Pectinas/química , Proteínas de Plantas/análisis , Raíces de Plantas/química , Secuencia de Aminoácidos , Glicoproteínas/química , Glicoproteínas/metabolismo , Espectrometría de Masas , Microscopía de Fuerza Atómica , Datos de Secuencia Molecular , Pectinas/metabolismo , Fragmentos de Péptidos/química , Fragmentos de Péptidos/metabolismo , Mapeo Peptídico , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Tripsina/metabolismoRESUMEN
Several high-resolution imaging techniques such as FESEM, TEM and AFM are compared with respect to their application on alginate hydrogels, a widely used polysaccharide biomaterial. A new AFM method applicable to RGD peptides covalently conjugated to alginate hydrogels is described. High-resolution images of RGD adhesion ligand distribution were obtained by labeling biotinylated RGD peptides with streptavidin-labeled gold nanoparticles. This method may broadly provide a useful tool for sECM characterization and design for tissue regeneration strategies.
Asunto(s)
Matriz Extracelular/química , Oro/química , Nanopartículas del Metal/química , Microscopía de Fuerza Atómica/métodos , Oligopéptidos/química , Alginatos/química , Materiales Biocompatibles/química , Biotina/química , Matriz Extracelular/ultraestructura , Hidrogeles/síntesis química , Hidrogeles/química , Microscopía Electrónica de Rastreo/métodos , Microscopía Electrónica de Transmisión , Estreptavidina/química , Andamios del Tejido/químicaRESUMEN
Images of high methoxyl orange pectin deposited from solution and high methoxyl sugar acid gels (HMSAG) were obtained by atomic force microscopy (AFM) in the tapping mode. For the first time, images of pectin deposited from water revealed that the transition from pectin networks to individual molecules or aggregates thereof occurred at concentrations between 6.5 and 13.1 microg/mL. At 6.5 microg/mL, shapes included rods, segmented rods, kinked rods, rings, branched molecules, and dense circular areas. At 13.1 microg/mL, all of these shapes were integrated into networks. These same structures were discernible in pectin high methoxyl sugar acid gels. Thus one might consider pectin networks in water at concentrations in excess of 10 microg/mL to be separate fluid precursors of networks in high methoxyl sugar acid gels. Examination of AFM images revealed that gels with "uniform" distribution of strands and pores between strands had higher gel strengths as measured by a penetrometer than gels in which strands were nonuniformly distributed and were separated by large and small spaces.
Asunto(s)
Pectinas/química , Citrus sinensis , Geles , Mecánica , Microscopía de Fuerza Atómica , Conformación Molecular , Soluciones , Azúcares Ácidos , AguaRESUMEN
This study was undertaken to evaluate the effect of modified citrus pectin (MCP) on the urinary excretion of toxic elements in healthy individuals. MCP is a reduced molecular weight pectin (weight-average molar mass = 15,400) that is mostly linear homogalacturonan with a 3.8% degree of esterification and approximately 10% rhamnogalacturonan II based on the presence of 2-keto-3-deoxy-octonic acid. Subjects ingested 15 g of MCP (PectaSol, EcoNugenics Inc., Santa Rosa, California 95407) each day for 5 days and 20 g on day 6. Twenty-four hour urine samples were collected on day 1 and day 6 for comparison with baseline. The urine samples were analysed for toxic and essential elements. In the first 24 h of MCP administration the urinary excretion of arsenic increased significantly (130%, p < 0.05). On day 6, urinary excretion was increased significantly for cadmium (150%, p < 0.05). In addition, lead showed a dramatic increase in excretion (560%, p < 0.08). This pilot trial provides the first evidence that oral administration of MCP increases significantly the urinary excretion of toxic metals in subjects with a 'normal' body load of metals. It is suggested that systemic chelation of toxic metals by MCP may in part be attributable to the presence of rhamnogalacturonan II, which has been shown previously to chelate metals.
Asunto(s)
Quelantes/farmacología , Citrus/química , Metales Pesados/orina , Pectinas/farmacología , Adulto , Arsénico/orina , Quelantes/química , Quelantes/aislamiento & purificación , Femenino , Humanos , Masculino , Persona de Mediana Edad , Pectinas/química , Pectinas/aislamiento & purificación , Proyectos Piloto , UrinálisisRESUMEN
Height and phase shift images of high methoxyl sugar acid gels (HMSAG) of pectin were obtained by atomic force microscopy in the tapping mode. Images revealed that pores in these gels were fluid and flattened out when measured as a function of time. These images revealed for the first time the structure of adsorbed sugar on pectin in the hydrated native gels and how the pectin framework is organized within these gels. Segmentation of images revealed that the underlying pectin framework contained combinations of rods, segmented rods, and kinked rods connected end to end and laterally. The open network of strands was similar to pectin aggregates from 5 mM NaCl solution imaged earlier by electron microscopy (Fishman et al., Arch. Biochem. Biophys. 1992, 294, 253). Area measurements revealed that the ratio of bound sugar to pectin was in excess of 100 to 1 (w/w). Furthermore, images indicated relatively small differences in the organization of native commercial citrus pectin, orange albedo pectin, and lime albedo pectin gels at optimal pH as determined in this study. The findings are consistent with earlier gel strength measurements of these gels. In addition, values of gel strength were consistent with values of molar mass and viscosity of the constituent pectins in that they increased in the same order. Finally, we demonstrated the advantage of simultaneous visualization of height and phase shift images for observing and quantitating the nanostructure of relatively soft gels which are fully hydrated with a buffer.