Enhanced wheat productivity in saline soil through the combined application of poultry manure and beneficial microbes.

BACKGROUND: Soil salinity is one of the major menaces to food security, particularly in dealing with the food demand of the ever-increasing global population. Production of cereal crops such as wheat is severely affected by soil salinity and improper fertilization. The present study aimed to examine the effect of selected microbes and poultry manure (PM) on seedling emergence, physiology, nutrient uptake, and growth of wheat in saline soil. A pot experiment was carried out in research area of Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad, Pakistan. Saline soil (12 dS m- 1 w/w) was developed by spiking using sodium chloride, and used in experiment along with two microbial strains (i.e., Alcaligenes faecalis MH-2 and Achromobacter denitrificans MH-6) and PM. Finally, wheat seeds (variety Akbar-2019) were sown in amended and unamended soil, and pots were placed following a completely randomized design. The wheat crop was harvested after 140 days of sowing. RESULTS: The results showed a 10-39% increase (compared to non-saline control) in agronomic, physiological, and nutritive attributes of wheat plants when augmented with PM and microbes. Microbes together with PM significantly enhanced seedling emergence (up to 38%), agronomic (up to 36%), and physiological (up to 33%) in saline soil as compared to their respective unamended control. Moreover, the co-use of microbes and PM also improved soil's physicochemical attributes and enhanced N (i.e., 21.7%-17.1%), P (i.e., 24.1-29.3%), and K (i.e., 28.7%-25.3%) availability to the plant (roots and shoots, respectively). Similarly, the co-use of amendments also lowered the Na+ contents in soil (i.e., up to 62%) as compared to unamended saline control. This is the first study reporting the effects of the co-addition of newly identified salt-tolerant bacterial strains and PM on seedling emergence, physiology, nutrient uptake, and growth of wheat in highly saline soil. CONCLUSION: Our findings suggest that co-using a multi-trait bacterial culture and PM could be an appropriate option for sustainable crop production in salt-affected soil.

Microbes-assisted phytoremediation of lead and petroleum hydrocarbons contaminated water by water hyacinth.

An experiment was carried out to explore the impact of petroleum hydrocarbons (PHs)-degrading microbial consortium (MC) on phytoremediation ability and growth of water hyacinth (WH) plants in water contaminated with lead (Pb) and PHs. Buckets (12-L capacity) were filled with water and WH plants, PHs (2,400 mg L-1) and Pb (10 mg L-1) in respective buckets. Plants were harvested after 30 days of transplanting and results showed that PHs and Pb substantially reduced the agronomic (up to 62%) and physiological (up to 49%) attributes of WH plants. However, the application of MC resulted in a substantial increase in growth (38%) and physiology (22%) of WH plants over uninoculated contaminated control. The WH + MC were able to accumulate 93% Pb and degrade/accumulate 72% of PHs as compared to initial concentration. Furthermore, combined use of WH plants and MC in co-contamination of PHs and Pb, reduced Pb and PHs contents in water by 74% and 68%, respectively, than that of initially applied concentration. Our findings suggest that the WH in combination with PHs-degrading MC could be a suitable nature-based water remediation technology for organic and inorganic contaminants and in future it can be used for decontamination of mix pollutants from water bodies.

Phytoremediation by aquatic macrophytes is a promising technique for the cleanup of environmental toxins from wastewater. To our knowledge, this is the first study reporting the integrated use of water hyacinth (WH) plants and a newly developed multi-trait microbial consortium for the simultaneous remediation of organic (i.e., petroleum hydrocarbons) and inorganic (i.e., lead) pollutants from the contaminated water. Findings of this study provide the basic but important information on the combined use of WH and microbes for remediation of mix pollution from water bodies.

Recent progress in CRISPR-based bioengineering of microbial cell factories for important nutraceuticals synthesis.

Nutraceuticals are defined as food or food components with therapeutic capabilities that have few side effects and are regarded as a natural therapy for preventing the onset of several life-threatening illnesses. The use of microbial cell factories to produce nutraceuticals is considered to be sustainable and promising for meeting market demand. Among the diverse strategies for optimizing microbial cell factories, the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system has emerged as a valuable tool for gene integration, deletion, activation, and downregulation. With the advent of multiplexed and precise CRISPR strategies, optimized microbial cell factories are revolutionizing the yield of nutraceuticals. This review focuses on the development of highly adaptable CRISPR strategies to optimize the production in microbial cell factories of some important nutraceuticals (belonging to the class of carotenoids, flavonoids, stilbenoids, polysaccharides, and nonprotein amino acids). Further, we highlighted current challenges related to the efficiency of CRISPR strategies and addressed potential future directions to fully harness CRISPR strategies to make nutraceutical synthesis in microbial cell factories an industrially favorable method.

Bacterial metabolic engineering for the production of second-generation (2 G) bioethanol and biobutanol; a review.

The second generation (2 G) biofuels were introduced to solve the issues associated with first-generation biofuel (dependency on food materials) and fossil fuels, such as reservoirs diminution, high demand, price fluctuation, and lethal greenhouse gases emission. Butanol and ethanol are the main 2 G biofuels. They are used as a disinfectant, antiseptic, and chemical solvent in the pharmaceutical, plastic, textiles, cosmetics, and fuel industries. Currently, their bacterial biological production from lignocellulosic material at the industrial level with primitive microorganisms is under development and not economical and qualitative compatible as compared to that of fossil origin, due to the slow growth rate, low titer, recalcitrant nature of lignocellulose, strain intolerance to a higher amount of butanol and ethanol, and strain inability to tolerate inhibitors accumulated during pretreatment of lignocellulosic materials. Therefore, metabolic engineering strategies such as redirection of carbon flux, knocking out competing pathways, enhancing strain robustness and wide range of substrate utilization ability, and overexpression of enzymes involved in their biological synthesis have been applied to bacteria for enhancing their ability for 2 G ethanol and butanol production in a highly cost-effective amount from lignocellulosic materials. Herein, we summarized and reviewed the progress in metabolic engineering of bacterial species such as Clostridium spp,Escherichia coli, and Zymomonas mobilis for the synthesis of 2 G butanol and ethanol, especially from lignocellulosic materials.

Enhancing water stress tolerance of bread wheat during seed germination and seedling emergence: caffeine-induced modulation of antioxidative defense mechanisms.

Better crop stand establishment, a function of rapid and uniform seedling emergence, depends on the activities of germination-related enzymes, which is problematic when there is insufficient soil moisture. Different ways are in practice for counteracting this problem, including seed priming with different chemicals, which are considered helpful in obtaining better crop stand establishment to some extent through improved seed germination and seedling emergence. In this growth room experiment, caffeine was used as a seed priming agent to improve germination under moisture scarcity. Polyethylene glycol-8000 (18%) was added to Hoagland's nutrient solution to create drought stress (-0.65 MPa). The experiment was arranged in a completely randomized design (CRD), having four replications of each treatment. A newly developed wheat genotype SB-1 was used for the experimentation. Different doses of caffeine, i.e., 4 ppm, 8 ppm, 12 ppm, and 16 ppm, including no soaking and water soaking, were used as seed priming treatments. Water deficit caused oxidative stress and adversely affected the seed germination, seedling vigor, activities of germination enzymes, photosynthetic pigments, and antioxidative defense mechanism in roots and shoots of seedlings. Caffeine seed priming ameliorated the negative effects of water deficit on seed germination and seedling vigor, which was attributed to the reduction in lipid peroxidation and improvement in the activities of germination-related enzymes like glucosidase, amylase, and protease. Conclusively, seed priming with 12 ppm caffeine outperformed the other treatments and hence is recommended for better crop stand establishment under conditions of soil moisture deficit.

Biofilm producing plant growth promoting bacteria in combination with glycine betaine uplift drought stress tolerance of maize plant.

Introduction: The escalating threat of drought poses a significant challenge to sustainable food production and human health, as water scarcity adversely impacts various aspects of plant physiology. Maize, a cornerstone in staple cereal crops, faces the formidable challenge of drought stress that triggers a series of transformative responses in the plant. Methods: The present study was carried out in two sets of experiments. In first experiment, drought stress was applied after maintaining growth for 45 days and then irrigation was skipped, and plant samples were collected at 1st, 3rd and 6th day of drought interval for evaluation of changes in plant growth, water relation (relative water content) and antioxidants activity by inoculating indigenously isolated drought tolerant biofilm producing rhizobacterial isolates (Bacillus subtilis SRJ4, Curtobacterium citreum MJ1). In the second experiment, glycine betaine was applied as osmoregulator in addition to drought tolerant PGPR to perceive modulation in photosynthetic pigments (Chlorophyll a and b) and plant growth under varying moisture stress levels (100, 75 and 50% FC). Results and discussion: Results of the study revealed upsurge in root and shoot length, fresh and dry biomass of root and shoot besides increasing chlorophyll contents in water stressed inoculated plants compared to uninoculated plants. Glycine betaine application resulted in an additional boost to plant growth and photosynthetic pigments, when applied in combination with bacterial inoculants. However, both bacterial inoculants behaved differently under drought stress as evident from their biochemical and physiological attributes. Isolate SRJ4 proved to be superior for its potential to express antioxidant activity, leaf water potential and relative water contents and drought responsive gene expression while isolate MJ1 showed exclusive increase in root dry biomass and plant P contents. Though it is quite difficult to isolate the bacterial isolates having both plant growth promoting traits and drought tolerance together yet, such biological resources could be an exceptional option to be applied for improving crop productivity and sustainable agriculture under abiotic stresses. By exploring the combined application of PGPR and glycine betaine, the study seeks to provide insights into potential strategies for developing sustainable agricultural practices aimed at improving crop resilience under challenging environmental conditions.

Therapeutic potential of boswellic acids: an update patent review (2016-2023).

INTRODUCTION: Boswellic acids (BAs) are a group of pentacyclic triterpenoids of the ursane and oleanane type. They have shown very interesting biological properties that have led to the development of a number of synthesis protocols. Both natural BAs and their synthetic derivatives may be useful in the treatment of a variety of cancers, viral infections and inflammatory diseases. AREAS COVERED: This review covers patents relating to the therapeutic activities of natural BAs and their synthetic derivatives. The latest patented studies of boswellic acids (are summarized by using the keywords 'boswellic acid,' in SciFinder, PubMed, and Google Patents and databases in the year from 2016 to 2023. EXPERT OPINION: Boswellic acids have shown potent antiviral, anticancer and anti-inflammatory potential. Few BAs analogues have been prepared by modification at the C24-CO2H functional groups. In particular, the C-24 amide and amino analogues have shown enhanced anticancer effects compared to the parent AKBA. In addition, BAs have the ability to form conjugates with other antiviral, anti-inflammatory and anticancer drugs that synergistically enhance their biological efficacy. In addition, this conjugation strategy will increase the solubility and bioavailability of BAs, which is one of the most important issues in the development of BAs.

Carbon and nitrogen stable isotope compositions provide new insights into the phenotypic plasticity of the invasive species Carpobrotus sp. pl. in different coastal habitats.

The genus Carpobrotus N.E.Br. comprises several aggressive invasive species that threaten biodiversity in coastal areas worldwide. We studied the phenotypic plasticity of Carpobrotus sp. pl. invading four coastal habitats in the north-western Iberian Peninsula (coastal cliffs, disturbed areas, dunes and coastal forests). We measured morphological traits and carbon (δ13C) and nitrogen (δ15N) stable isotope compositions of Carpobrotus sp. pl. individuals collected in each habitat. Our results indicated that leaf carbon content (% C) and dry shoot weight were higher on cliffs and lower in mixed forests. In contrast, leaf hydration was higher in mixed forests and lower on cliffs. Leaf nitrogen content (% N) was higher in forests, which might be due to the presence of Acacia longifolia, an alien tree that accumulates N in the soil through symbiotic associations with N fixing bacteria. Differences in δ15N showed the use of different N sources in each habitat. Values were higher in disturbed areas with greater human activity and lower on cliffs and forests. δ13C was higher in cliffs and dunes, suggesting CAM activity where drought and salinity are more intense. Water use efficiency (iWUE) and δ13C were higher on cliffs and dunes, suggesting an adaptation and high tolerance of Carpobrotus sp. pl. to unfavourable conditions such as drought or salinity in the invaded areas.

Efficient detoxification of textile wastewater by applying Chenopodium album nanoparticles and its application in simulated metal-bearing effluents removal.

Large-scale pollution of water and soils bodies is associated with the discharge of the untreated textile industry effluents. Halophytes grows on saline lands and accumulate secondary metabolites and other stress protective compounds. Utilization of Chenopodium album (halophytes) to synthesize zinc oxide (ZnO) and their efficiency to treat different concentrations of textile industry waste water is proposed in this study. Potential of nanoparticles textile industry waste water effluents was also analyzed by exposing different concentrations of nanoparticles (0 (control), 0.2, 0.5, 1 mg) and time intervals of 5, 10, and 15 days. The absorption peaks by UV region, FTIR and SEM analysis were used characterized on ZnO NPs for the first time. FTIR analysis showed the preens of various functional groups and vital phytochemicals that can play its role in the formation of nanoparticles that can be used for trace elements removal and bioremediation. SEM analysis indicated that the pure ZnO NPs synthesis ranged from 30 to 57 nm. Results shows that the green synthesis of halophytic nanoparticles presents maximum removal capacity after 15 days exposure to 1 mg of ZnO NPs. Hence, the prepared ZnO Nps from halophytes can be a viable solution for treating the textile industry effluents before they are discharged into water bodies for sustainable environmental growth and environmental safety.

Phytoremediation of Potentially Toxic Elements from Contaminated Saline Soils Using Salvadora persica L.: Seasonal Evaluation.

Plants in coastal ecosystems are primarily known as natural sinks of trace metals and their importance for phytoremediation is well established. Salvadora persica L., a medicinally important woody crop of marginal coasts, was evaluated for the accumulation of metal pollutants (viz. Fe, Mn, Cu, Pb, Zn, and Cr) from three coastal areas of Karachi on a seasonal basis. Korangi creek, being the most polluted site, had higher heavy metals (HM's) in soil (Fe up to 17,389, Mn: 268, Zn: 105, Cu: 23, Pb: 64.7 and Cr up to 35.9 mg kg-1) and S. persica accumulated most of the metals with >1 TF (translocation factor), yet none of them exceeded standard permissible ranges except for Pb (up to 3.1 in roots and 3.37 mg kg-1 in leaves with TF = 11.7). Seasonal data suggested that higher salinity in Clifton and Korangi creeks during pre- and post-monsoon summers resulted in lower leaf water (ΨWo) and osmotic potential at full turgor (ΨSo) and bulk elasticity (ε), higher leaf Na+ and Pb but lower extractable concentrations of other toxic metals (Cr, Cu, and Zn) in S. persica. Variation in metal accumulation may be linked to metal speciation via specific transporters and leaf water relation dynamics. Our results suggested that S. persica could be grown on Zn, Cr and Cu polluted soils but not on Pb affected soils as its leaves accumulated higher concentrations than the proposed limits.

Evaluating the Efficacy of Activated Carbon in Minimizing the Risk of Heavy Metals Contamination in Spinach for Safe Consumption.

Toxicity induced by heavy metals is a major concern in agriculture as it decreases crops' growth and yield and leads to the deterioration of food quality. Recently, activated carbon has been identified as a possible solution. It can potentially improve crop nutrition and immobilize heavy metals in soil. That is why a glasshouse trial was conducted to investigate the effects of sugarcane bagasse-derived biochar on spinach growth and the availability of cadmium (Cd) and chromium (Cr) in artificially contaminated soil. The soil was placed in pots and contaminated with Cd and Cr at a rate of 10 mg kg-1. Biochar was added to the soil at concentrations of 0 (control), 0 (contaminated control), 100, 150, and 200 g, and 10-day-old nursery spinach plants were transplanted to the pots. The results showed that applying 200 g of biochar significantly increased shoot weight (235 g), soil pH, electrical conductivity, and organic matter. The highest levels of Cd (27.71 mg kg-1) and Cr (20.44 mg kg-1) were observed in the contaminated control pots, while the lowest levels of Cd (16.80 mg kg-1) and Cr (9.80 mg kg-1) were found in pots treated with 200 g of biochar (2%). Similarly, the highest levels of Cd (35.80 mg kg-1) and Cr (40.24 mg kg-1) in the roots were found in the contaminated control pots, while the lowest levels of Cd (19.26 mg kg-1) and Cr (21.34 mg kg-1) were observed in pots treated with 200 g of biochar. Biochar application at a rate of 2% can immobilize Cd and Cr in the soil and improve chlorophyll contents, carotenoids, photosynthetic rate, transpiration rate, and stomatal conductance in spinach in Cd- and Cr-contaminated soils. Further long-term field studies will be necessary to determine the feasibility of applying biochar as an organic amendment for enhancing spinach growth and reducing Cd and Cr bioavailability in contaminated soil.

Plant Responses to Biotic and Abiotic Stresses: Crosstalk between Biochemistry and Ecophysiology.

Biotic and abiotic stresses, such as drought, salinity, extreme temperatures (cold and heat) and oxidative stress, are often interrelated; these conditions singularly or in combination induce cellular damage [...].

Comparative Plasticity Responses of Stable Isotopes of Carbon (δ13C) and Nitrogen (δ15N), Ion Homeostasis and Yield Attributes in Barley Exposed to Saline Environment.

Salinity is a major threat to agricultural productivity worldwide. The selection and evaluation of crop varieties that can tolerate salt stress are the main components for the rehabilitation of salt-degraded marginal soils. A field experiment was conducted to evaluate salinity tolerance potential, growth performance, carbon (δ13C) and nitrogen isotope composition (δ15N), intrinsic water use efficiency (iWUE), harvest index, and yield stability attributes in six barley genotypes (113/1B, 59/3A, N1-10, N1-29, Barjouj, Alanda01) at three salinity levels (0, 7, and 14 dS m-1). The number of spikes m-2 was highest in Alanda01 (620.8) while the lowest (556.2) was exhibited by Barjouj. Alanda01 produced the highest grain yield (3.96 t ha-1), while the lowest yield was obtained in 59/3A (2.31 t ha-1). Genotypes 113/1B, Barjouj, and Alanda01 demonstrate the highest negative δ13C values (-27.10‱, -26.49‱, -26.45‱), while the lowest values were obtained in N1-29 (-21.63‱) under salt stress. The δ15N was increased (4.93‱ and 4.59‱) after 7 and 14 dS m-1 as compared to control (3.12‱). The iWUE was higher in N1-29 (144.5) and N1-10 (131.8), while lowest in Barjouj (81.4). Grain protein contents were higher in 113/1B and Barjouj than other genotypes. We concluded that salt tolerant barley genotypes can be cultivated in saline marginal soils for food and nutrition security and can help in the rehabilitation of marginal lands.

Role of vitamin C in preventing of COVID-19 infection, progression and severity.

Vitamin C stands as an essential water-soluble vitamin, antioxidant and has been shown to enhance immunity. SARS-CoV-2 has been spreading rapidly across the worldwide, several cellular processes of innate and adaptive immunity are aided by vitamin C, which strengthens the immune system overall. Multiple lines of evidence in the literature associate vitamin C with antioxidant, anti-inflammatory, anticoagulant and immunomodulatory actions. Pneumonia and sepsis patients had poor ascorbic acid status and high oxidative stress, according to many studies. Pneumonia patients who get vitamin C may have less severe symptoms and a longer course of the illness if they do. To standardize plasma levels in sepsis patients, gram measurements of the vitamin must be administered intravenously (IV). This intervention has been shown in a few trials to reduce mortality. COVID-19 management in China and the United States has exhibited remarkable results when using a high percentage of intravenous vitamins C. It's acceptable to include vitamin C in the COVID-19 treatment protocol as a secondary measure based on the current active clinical studies looking at the impact of vitamin C on the management of COVID-19. Patients with hypovitaminosis C or severe respiratory illnesses, such as COVID-19, may benefit from taking vitamin C, due to its good safety profile, simplicity of use, and potential for rapid production scaling. The study's goal was to see whether high dosage intravenous vitamin C had any impact on individuals with severe COVID-19 (HDIVC). Finally we discuss recent research that has been published on the efficacy of vitamin C administration in the treatment of viral infection and life-threatening conditions. The purpose of this manuscript is to summarise existing research on the efficacy of vitamin C as a treatment for COVID-19 and to discuss possible explanations for why it may work in some individuals but not in others.

Appraising growth, daily intake, health risk index, and pollution load of Zn in wheat (Triticum aestivum L.) grown in soil differentially spiked with zinc.

Zinc (Zn) is a vital nutrient element required for plants normal growth and development. It performs imperative functions in numerous metabolic pathways in the plants. However, potentially noxious levels of Zn in terrestrial environment can lead to inhibited photosynthesis, growth, respiratory rate and imbalanced mineral nutrition. In micronutrient malnutrition, Zn deficiency is a global human health problem owing to the human dependence on cereals grains especially wheat-based diet. Therefore, this study investigated the Zn uptake efficacy in Triticum aestivum that is grown under two different doses (100 g/kg or 200 g/kg) of various soil amendments in both pot and field experimentation. Results of this study revealed that mean Zn concentration in different wheat varieties and treatments were varied from 1.53 to 6.03 mg/kg, 11.27 to 40.65 mg/kg, 11.28 to 39.93 mg/kg, and 11.32 to 37.70 mg/kg in amended soil, root, shoot, and grains, respectively. All observed Zn values in soil and wheat parts were lower than the FAO/WHO standards. Zinc values observed for pollution load index (0.034-0.134 mg/kg), daily intake (0.00492-0.01533 mg/kg), and health risk (0.0164-0.0570 mg/kg) index were lower than 1 except bio-concentration factor. Bio-concentration factor (5.076-10.165 mg/kg) revealed that DHARABI-11 variety showed maximum Zn uptake efficacy in farmyard manure treatment. The daily intake and health risk index values also showed that Zn level in grains is safe for inhabitants consumption. Overall, study recommended that these organic amendments are a good source of fertilizers, essentially required for the sustainable management of soil and increases the Zn accumulation in wheat grains which can ultimately reduce the Zn malnutrition in human food chain.

Effect of slow release nitrogenous fertilizers and biochar on growth, physiology, yield, and nitrogen use efficiency of sunflower under arid climate.

Sunflower plants need nitrogen consistently and in higher amount for optimum growth and development. However, nitrogen use efficiency (NUE) of sunflower crop is low due to various nitrogen (N) losses. Therefore, it is necessary to evaluate the advanced strategies to minimize N losses and also improve sunflower productivity under arid climatic conditions. A field trial was conducted with four slow release nitrogenous fertilizers [SRNF (bacterial, neem, and sulfur-coated urea and N loaded biochar)] and three N levels (100% = 148 kg N ha-1, 80% = 118 kg N ha-1, and 60% = 89 kg N ha-1) of recommended application (100%) for sunflower crop under arid climatic conditions. Results showed that neem-coated urea at 148 kg N ha-1 significantly enhanced crop growth rate (CGR) (19.16 g m-2 d-1) at 60-75 days after sowing (DAS); leaf area index (2.12, 3.62, 5.97, and 3.00) at 45, 60, 75, and 90 DAS; and total dry matter (14.27, 26.29, 122.67, 410, and 604.33 g m-2) at 30, 45, 60, 75, and 90 DAS. Furthermore, higher values of net leaf photosynthetic rate (25.2 µmol m-2 s-1), transpiration rate (3.66 mmol s-1), and leaf stomatal conductance (0.39 mol m-2 s-1) were recorded for the same treatment. Similarly, neem-coated urea produced maximum achene yield (2322 kg ha-1), biological yield (9000 kg ha-1), and harvest index (25.8%) of the sunflower crop. Among various N fertilizers, neem-coated urea showed maximum NUE (20.20 kg achene yield kg-1 N applied) in comparison to other slow release N fertilizers. Similarly, nitrogen increment N60 showed maximum NUE (22.40 kg grain yield kg-1 N applied) in comparison to N80 and N100. In conclusion, neem-coated urea with 100% and 80% of recommended N would be recommended for farmers to get better sunflower productivity with sustainable production and to reduce the environmental nitrogen losses.

Assessment of cobalt in wheat grains as affected by diverse fertilizers: implications for public health.

Present study was conducted to check the heavy metal content in wheat treated with municipal solid waste, Although municipal solid waste was enriched with organic nutrient, micronutrients, and macro-nutrients, considerable amount of cobalt was also witnessed in municipal solid waste that s why pot experiment was executed. The concentration of cobalt in different parts of wheat (root, shoot, and grain) was analyzed by atomic absorption spectrophotometer (AA-6300 Shimadzu Japan). Highest concentration of cobalt in root, shoot, and grain of wheat was observed in the range of 0.91-1.02 mg/kg, 0.92-1.04 mg/kg, and 0.93-1.00 mg/kg, respectively, under the influence of different fertilizer's used, while in field experiment, level of Co was greater in roots followed by shoots and grain. The metal concentration in wheat grown in field was ranged from 0.67 to 0.72 mg/kg for roots, 0.64 to 0.71 mg/kg for shoots, and 0.66 to 1.71 mg/kg for grains. Concentrations of cobalt were found below the permissible limits suggested by FAO/WHO. Various indices (PLI, BCF, DIM, HRI) were calculated and results showed that PLI was above 1 indicating that metal was causing pollution in treatments while value of BCF, DIM, and HRI was within the permissible range. Higher Co content in wheat may cause damage to the pancreatic cells of animals, cause respiratory problems, and effects their kidney, liver, and lungs, if exposure is for long period through feed.

Effect of slow-release nitrogenous fertilizers on dry matter accumulation, grain nutritional quality, water productivity and wheat yield under an arid environment.

Slow release nitrogenous fertilizers can improve crops production and reduce the environmental challenges in agro-ecosystem. There is a need to test the efficiency and performance under arid climatic conditions. The study investigates the effect of slow-release fertilizers (urea, neem coated urea (NCU), sulfur coated urea (SCU) and bioactive sulfur coated urea (BSCU)) on the growth, productivity and grain nutritional qualities of wheat crop. Slow-release fertilizers (SRF) with nitrogen levels (130,117,104 and 94 kg ha-1) were applied with equal splits at sowing, 20 and 60 days after sowing (DAS). Research showed that the BSCU with 130 kg ha-1 increased dry matter accumulation (1989 kg ha-1) after anthesis and grain yield 4463 kg ha-1. The higher plant height (102 cm) was attained by 130 kg N ha-1 SCU while the minimum (77.67 cm) recorded for 94 kg N ha-1 as urea source. Maximum grain NPK concentrations (3.54, 0.66 and 1.07%) were recorded by BSCU 130 kg N ha-1 application. While, the minimum NPK (0.77, 0.19 and 0.35%) were observed by Urea 94 kg N ha-1. The high irrigation water use efficiency (WUE) recorded (20.92 kg ha-1 mm-1) and a crop index of 25.52% by BSCU 130 kg N ha-1 application. Research findings show that generally all SRF but particularly BSCU proved effective and can be recommended for wheat crop under arid environment.

Assessment of Heavy Metal Accumulation in Soil and Garlic Influenced by Waste-Derived Organic Amendments.

In South Asia, the high costs of synthetic fertilizers have imposed research on alternative nutrient inputs. We aimed to identify potential trace elements (PTE) present in some organic manure that might be a source of environmental pollutions and risk to public health following consumption. The study aims to evaluate how different organic waste (poultry waste, PW; press mud, PM; and farmyard manure, FYM) influences the heavy metal migration in soil, the accumulation in garlic, and their potential health risks. Organic waste caused a higher accumulation of certain metals (Zn, Cu, Fe, and Co), whereas Mn, Cd, Cr, and Pb were in lower concentrations in soil. Amendments of soil with PM revealed a higher accumulation of Cd, Cr, Fe, and Pb, whereas PW resulted in Cu and Zn accumulation in garlic. Treatment of soil with FYM exhibited higher metal concentration of Co and Mn. An environmental hazard indices study revealed that pollution load index (PLI) was highest for Cu following treatment with PM. The health risk index (HRI) was greater for Cd following amendment with PM. Maximum daily intake of metals (DIM) was observed for Zn after treatment with PW. The Pb exhibited maximum bioconcentration factor (BCF) in PM-amended plants. Based on these findings, we concluded that garlic grown on contaminated soil with organic waste may pose serious health hazards following consumption.

Assessment of Uptake, Accumulation and Degradation of Paracetamol in Spinach (Spinacia oleracea L.) under Controlled Laboratory Conditions.

The occurrence and persistence of pharmaceuticals in the food chain, particularly edible crops, can adversely affect human and environmental health. In this study, the impacts of the absorption, translocation, accumulation, and degradation of paracetamol in different organs of the leafy vegetable crop spinach (Spinacia oleracea) were assessed under controlled laboratory conditions. Spinach plants were exposed to 50 mg/L, 100 mg/L, and 200 mg/L paracetamol in 20% Hoagland solution at the vegetative phase in a hydroponic system. Exposed plants exhibited pronounced phytotoxic effects during the eight days trial period, with highly significant reductions seen in the plants' morphological parameters. The increasing paracetamol stress levels adversely affected the plants' photosynthetic machinery, altering the chlorophyll fluorescence parameters (Fv/Fm and PSII), photosynthetic pigments (Chl a, Chl b and carotenoid contents), and composition of essential nutrients and elements. The LC-MS results indicated that the spinach organs receiving various paracetamol levels on day four exhibited significant uptake and translocation of the drug from roots to aerial parts, while degradation of the drug was observed after eight days. The VITEK® 2 system identified several bacterial strains (e.g., members of Burkhulderia, Sphingomonas, Pseudomonas, Staphylococcus, Stenotrophomonas and Kocuria) isolated from spinach shoots and roots. These microbes have the potential to biodegrade paracetamol and other organic micro-pollutants. Our findings provide novel insights to mitigate the risks associated with pharmaceutical pollution in the environment and explore the bioremediation potential of edible crops and their associated microbial consortium to remove these pollutants effectively.