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1.
Clin Oral Investig ; 25(8): 5009-5024, 2021 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-33638052

RESUMEN

OBJECTIVE: The aim of the present study was to evaluate the in vitro biocompatibility of Theracal PT, Theracal LC, and MTA Angelus, considered as bioactive materials used for vital pulp treatment, on human dental pulp stem cells (hDPSCs). MATERIALS AND METHODS: Human dental pulp stem cells (hDPSCs) were isolated from third molars, and material eluates were prepared (undiluted, 1:2, and 1:4 ratios). The hDPSC cytotoxicity, adhesion, morphology, viability, and cell migration were assessed. The mineralization nodule formation was determined by Alizarin red S staining (ARS). The odonto/osteogenic differentiation potential was assessed by osteo/odontogenic marker expression real-time qPCR. The chemical composition and ion release of the vital pulp materials were determined by energy dispersive X-ray (EDX) and inductively coupled plasma-mass spectrometry (ICP-MS), respectively. Statistical differences were assessed by ANOVA and Tukey's test (p < 0.05). RESULTS: The three vital pulp materials showed variable levels of calcium, tungsten, silicon, and zirconium release and in their chemical composition. Cytocompatibility assays revealed higher hDPSC viability and migration rates when treated with Theracal PT than with Theracal LC. The lowest cell adhesion and spreading were observed in all Theracal LC-treated groups, whereas the highest were observed when treated with MTA. Theracal PT and MTA promoted the upregulation of DSPP and RUNX2 gene expression (p < 0.05). After 21 days, both MTA Angelus and Theracal PT-treated cells exhibited a significantly higher mineralized nodule formation than the negative control (p < 0.05). CONCLUSIONS: This study demonstrates the favorable in vitro cytocompatibility and bioactive properties of the recently introduced Theracal PT and the well-established MTA Angelus on hDPSCs, as opposed to Theracal LC. More studies, including in vivo animal testing are suggested before these new formulations might be used in the clinical setting. CLINICAL RELEVANCE: Theracal PT is a new material that could be clinically suitable for vital pulp therapy. Further studies considering its biocompatibility and bioactivity are necessary.


Asunto(s)
Osteogénesis , Células Madre , Compuestos de Aluminio , Compuestos de Calcio/farmacología , Pulpa Dental , Combinación de Medicamentos , Humanos , Ensayo de Materiales , Óxidos , Silicatos/farmacología
2.
Curr Oncol ; 24(3): e244-e250, 2017 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-28680293

RESUMEN

We report a case of a recurrent clear cell meningioma (ccm) in the frontal lobe of the brain of a 67-year-old man. The patient developed three recurrences: at 3, 10, and 12 years after his initial surgery. Histopathology observations revealed a grade 2 ccm with positivity for vimentin and epithelial membrane antigen. Expression of E-cadherin was positive only in the primary tumour and in the first available recurrence. Fluorescence in situ hybridization analyses demonstrated 1p and 14q deletions within the last recurrence. Multiplex ligation-dependent probe amplification studies revealed a heterozygous partial NF2 gene deletion, which progressed to total loss in the last recurrence. The last recurrence showed homozygous deletions in CDKN2A and CDKN2B. The RASSF1 gene was hypermethylated during tumour evolution. In this report, we show the genetic alterations of a primary ccm and its recurrences to elucidate their relationships with the changes involved in the progression of this rare neoplasm.

4.
Br J Pharmacol ; 152(8): 1272-82, 2007 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-17934513

RESUMEN

BACKGROUND AND PURPOSE: Our aim was to examine the effect of demethylnobiletin on various experimental models of delayed-type hypersensitivity (DTH) reactions and to determine its influence on the mediators and enzymes involved in these reactions. EXPERIMENTAL APPROACH: DTH was induced in mice by oxazolone, dinitrofluorobenzene (DNFB) and sheep red blood cells (SRBC). The effect of demethylnobiletin on the ensuing DTH was studied, especially in relation to oedema formation, cell infiltration and tissue damage. Its activity on different mediators implicated in DTH reactions was also determined and its effect on nitric oxide synthase (NOS)-2 analysed. Finally, its influence on T lymphocyte proliferation, apoptosis and caspase 3 activity was tested. KEY RESULTS: DTH reactions were all reduced by demethylnobiletin. The experimental results suggest that the compound may act by reducing cell infiltration and by suppressing mediators such as interleukin-2 (IC50=1.63 microM), interleukin-4 (IC50=2.76 microM), tumour necrosis factor-alpha (IC50=0.66 microM), interferon-gamma (IC50=1.35 microM), and interleukin-1 beta (46% at 2.5 microM) and by concomitantly increasing the production of the anti-inflammatory cytokine, interleukin-10. In addition, while demethylnobiletin affected nitric oxide production, it did not modify NOS-2 expression. Finally, demethylnobiletin inhibited proliferation of T cells and induced their apoptosis. CONCLUSIONS AND IMPLICATIONS: Demethylnobiletin decreased DTH reactions induced by various agents. This finding, along with the fact that the compound has a low toxicity and exhibits several other interesting properties, could pave the way for other structurally related citroflavonoids to be used as pharmacological agents in complementary therapies.


Asunto(s)
Citocinas/efectos de los fármacos , Flavonas/farmacología , Hipersensibilidad Tardía/tratamiento farmacológico , Mediadores de Inflamación/metabolismo , Linfocitos T/efectos de los fármacos , Animales , Apoptosis/efectos de los fármacos , Caspasa 3/efectos de los fármacos , Caspasa 3/metabolismo , Proliferación Celular/efectos de los fármacos , Citocinas/biosíntesis , Dinitrofluorobenceno , Modelos Animales de Enfermedad , Eritrocitos/efectos de los fármacos , Eritrocitos/metabolismo , Femenino , Regulación Enzimológica de la Expresión Génica/efectos de los fármacos , Humanos , Hipersensibilidad Tardía/fisiopatología , Ratones , Óxido Nítrico/biosíntesis , Óxido Nítrico Sintasa de Tipo II/efectos de los fármacos , Óxido Nítrico Sintasa de Tipo II/metabolismo , Oxazolona , Ovinos , Linfocitos T/metabolismo
5.
Clin Neuropathol ; 26(5): 224-31, 2007.
Artículo en Inglés | MEDLINE | ID: mdl-17907599

RESUMEN

Glioblastoma multiforme is the most common and most aggressive of the primary brain tumors. The mean survival of patients is 10-12 months. Conventional therapy of surgery, radiation and chemotherapy is largely palliative. Cytogenetically, karyotypes of glioblastomas are very complex with trisomy 7 and monosomy 10 as the most frequent abnormalities. A genetic alteration that is significantly more frequent in primary than in secondary glioblastomas, the latter arising from preceding low-grade gliomas, is epidermal growth factor receptor gene (EGFR) amplification, whereas TP-53 mutations are significantly more frequent in low-grade gliomas and secondary glioblastomas derived there- from. We report the histological and genetic study of two glioblastomas, one case arising de novo and the other case arising 3 years after a previously diagnosed anaplastic astrocytoma, with concurrent EGFR amplification and TP-53 mutation. These anomalies were initially deemed as mutually exclusive. However, a small percentage of cases have been found with both anomalies although at a significantly lower level than could be expected. We have analyzed these two cases cytogenetically and by molecular studies in order to detect additional alterations associated with this phenotype. Cytogenetically, both cases showed in common the monosomy of chromosomes 10 and 17. At the molecular level, a rare mutation of TP-53 was found in the secondary glioblastoma and hypermethylation of the promoter region of p16(INK4a) and p14(ARF) genes were observed in the primary and secondary glioblastoma, respectively.


Asunto(s)
Neoplasias Encefálicas/genética , Receptores ErbB/genética , Genes p53 , Glioblastoma/genética , Neoplasias Primarias Secundarias/genética , Astrocitoma/genética , Astrocitoma/metabolismo , Astrocitoma/patología , Neoplasias Encefálicas/metabolismo , Neoplasias Encefálicas/patología , Femenino , Amplificación de Genes , Glioblastoma/metabolismo , Glioblastoma/patología , Humanos , Inmunohistoquímica , Hibridación Fluorescente in Situ , Masculino , Persona de Mediana Edad , Mutación , Neoplasias Primarias Secundarias/metabolismo , Neoplasias Primarias Secundarias/patología
6.
Clin Neuropathol ; 25(4): 193-9, 2006.
Artículo en Inglés | MEDLINE | ID: mdl-16866301

RESUMEN

Glioblastoma is the most common primary tumor of the central nervous system, but the underlying genetic changes that give rise to these tumors are still poorly understood. We report a primary glioblastoma with an unusual age of presentation. The patient was a 22-year-old man with a survival of 16 months. Morphological findings showed an increase of cellularity with positive GFAP and EGFR expression, increase of proliferate index, vascular hyperplasia with glomeruloid structures and necrosis. Molecular analysis showed EGFR amplification. No mutations of the TP53 or amplification of MDM2 and CDK4 were detected. Neither homozygous deletion of the 9p21 locus genes nor aberrant methylation were found. The cytogenetic study showed a clonal karyotype. The metaphases presented, among other anomalies, a small ring chromosome and double-minutes chromosomes. Using FISH and CGH techniques, it was found that the ring chromosome was a partial trisomy of chromosome 7, and the region implicated corresponded to 7p13-q21. Partial trisomies in glioblastoma could play an important role in defining those regions where genes implicated in this tumor process may be found. We studied the possible correlation of these findings with the tumoral phenotype.


Asunto(s)
Neoplasias Encefálicas/genética , Cromosomas Humanos Par 7 , Genes erbB-1/genética , Glioblastoma/genética , Cromosomas en Anillo , Adulto , Neoplasias Encefálicas/cirugía , Neoplasias Encefálicas/ultraestructura , Cromosomas Humanos Par 7/ultraestructura , Factor de Crecimiento Epidérmico/genética , Factor de Crecimiento Epidérmico/metabolismo , Amplificación de Genes , Proteína Ácida Fibrilar de la Glía/metabolismo , Glioblastoma/cirugía , Glioblastoma/ultraestructura , Humanos , Inmunohistoquímica , Cariotipificación , Masculino , Trisomía/patología
7.
Clin Neuropathol ; 24(5): 209-18, 2005.
Artículo en Inglés | MEDLINE | ID: mdl-16167544

RESUMEN

Glioblastoma multiforme (GBM) is characterized by intratumoral heterogeneity in both histomorphological and genetic changes, displaying a wide variety of numerical chromosome aberrations, the most common of which are trisomy 7 and monosomy 10. The amplification of the epidermal growth factor receptor (EGFR) gene is the most frequently reported genetic abnormality. The associations between these parameters and their implication in the tumoral progression are poorly understood. We performed simultaneous fluorescence in situ hybridization (FISH) with centromeric DNA probes for chromosomes 7 and 10 in smear preparations, and EGFR gene amplification by PCR from 25 cases of GBM. Trisomy/ polysomy for chromosome 7 was present in 76% of cases and monosomy 10 in 68%. Both alterations were associated in 56% of cases. The EGFR gene was amplified in 52% of tumors; in 44% associated with trisomy/ polysomy 7, and in 36% with monosomy 10. The three parameters were associated together in 28% of cases. Kaplan-Meier survival rate analysis demonstrated lower survival rates in patients with monosomy 10, trisomy 7, and monosomy associated with trisomy 7. The other combinations were not different in frequency in relation to survival. In the present study, trisomy/polysomy 7 and monosomy 10 have been found to be frequently associated. The combination of both anomalies is probably important in the tumorigenesis of glioblastoma. Moreover, this association is apparently independent of EGFR gene amplification, which could be a later event in this process.


Asunto(s)
Neoplasias Encefálicas/genética , Cromosomas Humanos Par 10/genética , Cromosomas Humanos Par 7/genética , Receptores ErbB/biosíntesis , Amplificación de Genes , Glioblastoma/genética , Adulto , Anciano , Biomarcadores de Tumor/análisis , Neoplasias Encefálicas/mortalidad , Neoplasias Encefálicas/patología , Niño , Receptores ErbB/genética , Femenino , Glioblastoma/mortalidad , Glioblastoma/patología , Humanos , Hibridación Fluorescente in Situ , Masculino , Persona de Mediana Edad , Reacción en Cadena de la Polimerasa , Pronóstico , Análisis de Supervivencia
8.
Neuroscience ; 297: 243-51, 2015 Jun 25.
Artículo en Inglés | MEDLINE | ID: mdl-25869624

RESUMEN

Glioblastoma (GBM) is the most frequent and malignant primary brain tumor. Conventional therapy of surgical removal, radiation and chemotherapy is largely palliative. Major vault protein (MVP), the main component of the vault organelle has been associated with multidrug resistance by reducing cellular accumulation of chemotherapeutic agents. With regard to cancer, MVP has been shown to be overexpressed in drug resistance development and malignant progression. The aim of the present study was to evaluate the MVP gene dosage levels in 113 archival samples from GBM and its correlation with patients' survival and epidermal growth factor receptor (EGFR) and phosphatase and tensin homolog (PTEN) gene dosages. Fluorescent in situ hybridization revealed polysomy of chromosome 7 in 76.1% of the GBMs and EGFR amplification in a 64.6% of the tumors. Genetic status of EGFR, PTEN and MVP copies was determined by multiplex ligation-dependent probe amplification (MLPA) technique. 31% of the tumors showed the EGFR is variant III mutation (EGFRvIII) mutation and 74.3% of them presented amplification of MVP gene. Amplification of EGFR and MVP was found in a 63.7% and 56.6% of the GBM, respectively. An inverse correlation between MVP and PTEN dosage values was observed. Besides, an inverse relationship between the survival of the patients treated with chemotherapy and the levels of MVP copies was determined. In conclusion, our study reveals an important role of MVP, together with EGFRvIII and PTEN, in the progression of GBM and proposes it as a novel and interesting target for new treatment approaches.


Asunto(s)
Neoplasias Encefálicas/metabolismo , Receptores ErbB/metabolismo , Regulación Neoplásica de la Expresión Génica/genética , Glioblastoma/metabolismo , Fosfohidrolasa PTEN/metabolismo , Partículas Ribonucleoproteicas en Bóveda/metabolismo , Adulto , Anciano , Neoplasias Encefálicas/genética , Cromosomas Humanos Par 7/genética , Receptores ErbB/genética , Femenino , Glioblastoma/genética , Humanos , Masculino , Persona de Mediana Edad , Mutación/genética , Fosfohidrolasa PTEN/genética , Estadísticas no Paramétricas , Adulto Joven
9.
Virchows Arch ; 434(4): 307-14, 1999 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-10335941

RESUMEN

Uterine sarcomas are approximately 3% of all malignant uterine corpus tumours. Of these, the tumours that originate solely in the stromal elements of the uterine wall are infrequent and have not been well characterized cytogenetically. We report two cases of endometrial stromal sarcomas (ESS), one low grade and one high grade, diagnosed by conventional histology, immunocytochemistry, electron microscopy and cytogenetics. Morphologically clear-cut differential structures were seen at optical, immunohistochemical, and electron microscopic levels, permitting a clear differential diagnosis. The low-grade ESS expressed hormonal receptors and vimentin, whereas the high-grade ESS showed no hormone receptors, high Ki-67 activity, and occasional cytokeratin-positive cells. Ultrastructurally, no malignant epithelial differentiation was seen in the tumour cells, but cilia were found in both cases. Cytogenetic study of the low-grade ESS showed pseudodiploid karyotype with chromosomes 6 and 20 rearranged. The high-grade ESS showed a complex karyotype with clonal numerical and structural anomalies. The chromosomes involved in the structural rearrangements were 1, 3, 6, 7, 13, 14, 15, 17, 19, and 21.


Asunto(s)
Neoplasias Endometriales/patología , Sarcoma Estromático Endometrial/patología , Anciano , Biomarcadores de Tumor/análisis , Aberraciones Cromosómicas/genética , Bandeo Cromosómico , Trastornos de los Cromosomas , Cromosomas Humanos Par 20/genética , Cromosomas Humanos Par 6/genética , Terapia Combinada , Neoplasias Endometriales/química , Neoplasias Endometriales/genética , Neoplasias Endometriales/terapia , Resultado Fatal , Femenino , Humanos , Técnicas para Inmunoenzimas , Cariotipificación , Microscopía Electrónica , Persona de Mediana Edad , Sarcoma Estromático Endometrial/química , Sarcoma Estromático Endometrial/genética , Sarcoma Estromático Endometrial/terapia
10.
Cancer Genet Cytogenet ; 33(2): 291-7, 1988 Jul 15.
Artículo en Inglés | MEDLINE | ID: mdl-3164249

RESUMEN

A direct cytogenetic analysis was performed on tumor samples obtained from two patients with clinical and histopathologic diagnosis of primary peripheral neuroepithelioma. Both tumors presented the translocation t(11;22)(q24;q12). These results confirm those previously obtained by other authors and suggest a common histogenetic origin for this tumor with Ewing's sarcoma and Askin's tumor, in which the same translocation has been described.


Asunto(s)
Cromosomas Humanos Par 11 , Cromosomas Humanos Par 22 , Tumores Neuroectodérmicos Periféricos Primitivos/genética , Neoplasias de los Tejidos Blandos/genética , Translocación Genética , Niño , Bandeo Cromosómico , Femenino , Marcadores Genéticos , Humanos , Cariotipificación , Masculino , Persona de Mediana Edad , Tumores Neuroectodérmicos Periféricos Primitivos/patología , Neoplasias de los Tejidos Blandos/patología
11.
Cancer Genet Cytogenet ; 75(2): 150-2, 1994 Jul 15.
Artículo en Inglés | MEDLINE | ID: mdl-8055481

RESUMEN

We studied a case of adenoid cystic carcinoma. Cytogenetic analysis was performed on short-term culture, and the karyotype revealed only an abnormal cell line with the following changes: partial trisomy 5q, 6q deletion, monosomy of chromosome 9,der(10)t(10;15), a possible ring chromosome 22, and loss of the Y chromosome. The implication of chromosomes 6 and 9 is considered in relation to the karyotypic evolution of this type of tumor.


Asunto(s)
Carcinoma Adenoide Quístico/genética , Aberraciones Cromosómicas , Neoplasias de los Senos Paranasales/genética , Seno Esfenoidal , Adulto , Carcinoma Adenoide Quístico/patología , Humanos , Cariotipificación , Masculino , Neoplasias de los Senos Paranasales/patología , Seno Esfenoidal/patología
12.
Cancer Genet Cytogenet ; 122(2): 149-52, 2000 Oct 15.
Artículo en Inglés | MEDLINE | ID: mdl-11106829

RESUMEN

Atypical teratoid/rhabdoid tumor of the central nervous system is a rare childhood tumor with a distinct histologic appearance and an aggressive clinical course. Few tumors have been analyzed cytogenetically. The only consistent chromosomal abnormality identified in some of these tumors has been monosomy or deletions of chromosome 22; in others, a normal chromosome 22 was present. The authors report an atypical teratoid/rhabdoid neoplasm of the central nervous system with a novel complex rearrangement affecting chromosomes 6 and 11 as the sole anomaly. The involvement of region 11p15 could be important in the pathogenesis of this entity.


Asunto(s)
Neoplasias del Sistema Nervioso Central/genética , Aberraciones Cromosómicas , Cromosomas Humanos Par 11/genética , Cromosomas Humanos Par 6/genética , Tumor Rabdoide/genética , Teratoma/genética , Neoplasias del Sistema Nervioso Central/patología , Niño , Femenino , Humanos , Hibridación Fluorescente in Situ , Cariotipificación , Tumor Rabdoide/patología , Cromosomas en Anillo , Teratoma/patología , Translocación Genética
13.
Cancer Genet Cytogenet ; 70(1): 74-6, 1993 Oct 01.
Artículo en Inglés | MEDLINE | ID: mdl-8221617

RESUMEN

We studied a case of typical syncytial meningioma. Cytogenetic analysis of the tumor cells showed a karyotype with normal chromosomes 22 and only one anomaly, del(1)(p32). Cases of meningiomas with normal chromosomes 22 and other anomalies are rare, and it is difficult to correlate their histologic characteristics and biologic behavior.


Asunto(s)
Neoplasias Encefálicas/patología , Cromosomas Humanos Par 1 , Meningioma/patología , Adulto , Deleción Cromosómica , Femenino , Humanos
14.
Cancer Genet Cytogenet ; 96(1): 81-4, 1997 Jul 01.
Artículo en Inglés | MEDLINE | ID: mdl-9209474

RESUMEN

Medulloblastoma is the most common primitive neuroectodermal tumor (PNET) in children, but is very rare in adults. An isochromosome for the long arms of 17, i(17q), is found in about 30% of pediatric cases. Cytogenetic studies in adults are very scarce; only six cases have been described cytogenetically: three cases had normal karyotype, two were studied partially, and another presented only two clonal structural anomalies: del(9)(q12) and del(11)(q22). We studied the chromosomes from medulloblastoma in a 27-year-old woman and found one hypotetraploid stemline with clonal alterations. In the structural anomalies, chromosomes 3, 9, 12, and i(17q) were involved. Chromosome 9 presented a deletion in the long arm, del(9)(q13), with consequent loss of the 9q13-->qter region. This anomaly was similar to one found in a previous case. We suggest that the partial loss of the long arm of chromosome 9 may be a characteristic change of adult medulloblastoma.


Asunto(s)
Neoplasias Cerebelosas/genética , Deleción Cromosómica , Cromosomas Humanos Par 9/genética , Meduloblastoma/genética , Adulto , Femenino , Humanos , Cariotipificación
15.
Cancer Genet Cytogenet ; 85(2): 113-7, 1995 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-8548733

RESUMEN

Cytogenetic analyses of 10 cases of recurrent meningiomas growing in culture between 1-10 days are reported, of which seven showed benign morphology, one atypical, and two, malignant features. Normal karyotypes with nonclonal alterations were found in three cases, one case with only monosomy 22, and complex karyotypes in the remaining six. Four cases were hypodiploid, one pseudodiploid, and one hyperdiploid. The chromosomes most often involved in structural rearrangements were 1, 7, and 14 and the losses were chromosomes 7, 10, 14, 15, 18, and 22. Ring chromosome, dicentrics, double minutes, and association between satellites were found in one case. These complex karyotypes with hypodiploidy, structural rearrangements, and other markers in recurrent meningiomas may indicate aggressive tumor characteristics.


Asunto(s)
Neoplasias Encefálicas/genética , Aberraciones Cromosómicas , Trastornos de los Cromosomas , Meningioma/genética , Adulto , Anciano , Neoplasias Encefálicas/patología , Femenino , Humanos , Cariotipificación , Masculino , Meningioma/patología , Persona de Mediana Edad , Recurrencia
16.
Cancer Genet Cytogenet ; 90(1): 57-62, 1996 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-8780749

RESUMEN

Cytogenetic analysis of 8 cases of xenografted human osteosarcomas are reported, including six newly diagnosed and two recurrent tumors. Histologically five were osteoblastic, two were chondroblastic, and one was the microcellular type. All tumors were studied in short-term primary cultures between two and six days. Clonal and nonclonal abnormalities were present in the eight cases; four had a chromosome number in the hypotriploid range, two in the hyperdiploid, one in the hypodiploid, and one in the hypertetraploid range. All cases had complex karyotypes and the recognizable structural rearrangements clustered to chromosome arms 1p, 1q, 3p, 5p, 6q, 11p, 13p, 14p, 15q, 16p, 16q, 20q, 21p, and 22q. Seven cases presented double monosomy 17 and six tumors showed double minutes (dmin) or a homogeneously staining region (hsr). This fact has been described recently and its relation with the amplification of the MDM2 gene observed in osteosarcomas is as yet unknown.


Asunto(s)
Neoplasias Óseas/genética , Cromosomas Humanos Par 17 , Amplificación de Genes , Monosomía , Osteosarcoma/genética , Adolescente , Adulto , Animales , Neoplasias Óseas/patología , Niño , Cromosomas Humanos Par 17/ultraestructura , Femenino , Humanos , Cariotipificación , Masculino , Ratones , Ratones Desnudos , Persona de Mediana Edad , Trasplante de Neoplasias , Osteosarcoma/patología , Trasplante Heterólogo
17.
Cancer Genet Cytogenet ; 125(2): 119-24, 2001 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-11369054

RESUMEN

Deletion of 1p is associated with histological progression to meningiomas. Detection of this alteration may be a predicting factor for recurrences in this tumor. We present 8 meningiomas from four patients: the original tumor and the first recurrence in one patient, and the first and second recurrences in the other three were studied. We compared results of monosomy 22 and deletion of chromosome 1p with cytogenetic methods and fluorescence in situ hybridization (FISH) analysis obtained from slides of direct preparations, of cultured cells and slides of touch preparations. The cytogenetic study showed normal chromosome 22 and deletion on 1p32 in both samples of one patient; only monosomy 22 in both recurrences in another patient, and normal karyotypes with different non-clonal anomalies in the other tumors. However, with FISH analysis, monosomy 22 in both recurrences of three patients was demonstrated, as well as the loss of 1p in all tumors. These results were more evident in the analysis of direct and touch preparations than in those of cultured cells.


Asunto(s)
Deleción Cromosómica , Cromosomas Humanos Par 1/ultraestructura , Neoplasias Meníngeas/genética , Meningioma/genética , Recurrencia Local de Neoplasia/genética , Adulto , Anciano , Rotura Cromosómica , Cromosomas Humanos Par 22 , Femenino , Humanos , Hibridación Fluorescente in Situ , Cariotipificación , Masculino , Neoplasias Meníngeas/patología , Neoplasias Meníngeas/cirugía , Meningioma/patología , Meningioma/cirugía , Persona de Mediana Edad , Monosomía , Recurrencia Local de Neoplasia/patología , Células Tumorales Cultivadas/ultraestructura
18.
Cancer Genet Cytogenet ; 99(2): 121-5, 1997 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-9398866

RESUMEN

Cytogenetic analyses of four malignant mixed mesodermal tumors (MMMT) of the uterus are reported, of which one was of the homologous type and three of the heterologous. Karyotypic analyses were obtained in two cases from original tumors and in two cases from tumors xenotransplanted into nude mice. The karyotype of the homologous MMMT was normal in three different passages of a nude mice xenograft line established from the primary tumor. The heterologous tumors showed normal karyotype in one case and hyperdiploid and near triploid range with extensive numerical and structural rearrangements in two cases. Deletion of chromosome 1 at p32, and deletion of chromosome 11 at q13 were common markers in anomalous cases. The chromosomes most often involved in structural rearrangements were chromosomes 1, 9, 11, 12, 17, and 19. Double minutes, homogeneously staining regions, and telomeric association were also seen.


Asunto(s)
Tumor Mesodérmico Mixto/patología , Neoplasias Uterinas/patología , Anciano , Animales , Aberraciones Cromosómicas , Femenino , Humanos , Cariotipificación , Ratones , Persona de Mediana Edad , Tumor Mesodérmico Mixto/genética , Trasplante de Neoplasias , Neoplasias Uterinas/genética
19.
Clin Neuropathol ; 19(6): 259-67, 2000.
Artículo en Inglés | MEDLINE | ID: mdl-11128617

RESUMEN

Meningiomas may display benign (grade I), atypical (grade II) and anaplastic (grade III) histopathological findings. The cytogenetic studies strongly suggest that secondary changes (beyond loss of chromosome 22) appear to be associated with more atypical features and with greater clinical aggressivity. We studied 60 tumors from 52 patients. Histopathological features such as nuclear pleomorphism, nucleolar prominence, mitosis, necrosis, cellular density, PCNA labeling index, and karyotype have been evaluated. The distribution in histological grades was: 50% benign, 33% atypical and 17% anaplastic meningiomas. Nuclear pleomorphism and nucleolar prominence showed a progressive increase in grades I, II and III. Multifocal micronecrosis was considered a criterion of malignancy. A significant correlation was observed between PCNA-LI, mitotic index and grades. Complex karyotypes increased progressively: benign (34% of cases), atypical (45% of cases) and anaplastic (70% of cases). The most common numerical alterations were losses of chromosomes 10, 14, 18 and 22. The chromosomes most often involved in structural anomalies were: 1, 4, 7, 14 and 22. Telomeric associations were present in four cases and double minutes in two cases. Prognostic criteria for these tumors have been analyzed on the basis of these data.


Asunto(s)
Aberraciones Cromosómicas , Neoplasias Meníngeas/genética , Neoplasias Meníngeas/patología , Meningioma/genética , Meningioma/patología , Adulto , Anciano , Deleción Cromosómica , Cromosomas Humanos Par 22/genética , Análisis Citogenético , Femenino , Humanos , Cariotipificación , Masculino , Persona de Mediana Edad , Índice Mitótico , Pronóstico , Recurrencia
20.
Clin Neuropathol ; 8(3): 130-3, 1989.
Artículo en Inglés | MEDLINE | ID: mdl-2743649

RESUMEN

The cytogenetic findings, based on G-banding, in six meningiomas are reported. Normal karyotypes were found in three cases and monosomy of chromosome 22 in the remaining three. In one of these three cases, a malignant meningioma, several chromosomes were lost, gonosome Y included. The possible significance of the association of chromosome alterations in meningiomas with the histology of the tumor and its biological aggressivity is discussed.


Asunto(s)
Aberraciones Cromosómicas/patología , Cromosomas Humanos Par 22 , Cariotipificación , Neoplasias Meníngeas/genética , Meningioma/genética , Adulto , Trastornos de los Cromosomas , Humanos , Masculino , Neoplasias Meníngeas/patología , Meningioma/patología , Persona de Mediana Edad
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