[Examining interaction of phages with microorganisms by fluorometry and electro-orientation spectroscopy]. / Izuchenie vzaimodeistvii fagov i mikroorganizmov s ispol'zovaniem metodov fliuorimetrii i élektroorientatsionni spektroskopii.

Bacterial sensitivity to different various phages was examined by electro-orientation spectroscopy, fluorometry, and electron microscopy. The strains of Pseudomonas aeruginosa, Staphylococcus aureus, Yersinia pestis, Mycobacterium smegmatis, and Xanthomonas campestris were used. The fluorescence intensity of a membranotropic agent in the ANS-cell-phage system was shown to depend on the interaction of a bacterial virus and a microorganism. Fluorometric data correlated with electro-orientation spectroscopic findings. An analysis of the low-frequency site makes it possible to determine phage adsorption on the bacterial surface. The changes in electro-orientation effects at high frequencies suggest that there are barrier dysfunctions in the external membranes and that there is cellular phage reproductions. Whether fluorometry and electro-orientation spectroscopy can be further used for rapid identification of microorganisms by using phages is discussed.

[Properties of MTPN11 mycobacteriophage]. / Svoistva mikobakteriofaga MTPN11.

Some characteristics of the poorly studied phage MTPH11, which is used for identification of mycobacteria, are presented. The phage has an isometric head and a long noncontractile tail (B1 morphotype). The attachment apparatus of this phage includes a basal plate composed of two joint disks and a single tail fiber. The constant of phage adsorption on Mycobacterium smegmatis ATCC607 cells is 6.6 x 10(-9) ml/min. The latent infection period in the MTPH11-host strain 607 system in 65 min; phage progeny ranges from 30 to 40 virions per one cell. The constant of phage inactivation with a homologous antiserum is 50 min-1. The buoyant density of intact MTPH11 virion in CsCl amounts to 1.520 g/cm3. The phage is susceptible to chloroform, retains lytic activity within a pH range of 5 to 9, and is resistant to inactivating agents. The G + C content of the phage DNA is 63 mol%.

[Study of the interaction of MTPN11 mycobacteriophage with host cells based on electron microscopy, fluorimetry, and electro-oriented spectroscopy]. / Izuchenie vzaimodeistviia mikobakteriofaga MTPN11 s kletkoi-khoziainom na osnove élektronnoi mikroskopii, fluorimetrii i élektro-orientatsionnoi spektroskopii.

According to electron-microscopic data, various cells in the M. smegmatis ATCC607 population interact differently with phage MTPH11. Fluorometric studies of phage-host interactions were performed using a membranotropic fluorescent probe, 8-anilino-1-naphthalene sulfonate (ANS). Changes in the electric characteristics of mycobacterial cells infected with the phage were studied by electro-orientational (EO) spectroscopy. The problem of the employment of fluorometry and EO spectroscopy for rapid phage typing of mycobacteria is discussed.