Exploring mechanism of Huayu Pills combined with immunocheckpoint inhibitors in treating triple-negative breast cancer based on network pharmacology / 国际中医中药杂志

Objective:To explore the potential mechanism of Huayu Pills in the treatment of TNBC (triple-negative breast cancer) using network pharmacology.Methods:TCMSP (Traditional Chinese Medicine Systems Pharmacology), PubChem, STITCH and SwissTargetPrediction databases were used to analyze the potential targets of Huayu Pills. TNBC disease targets were screened based on the TCGA (The Cancer Genome Atlas) database. The drug-disease mapping target was constructed by PPI network, key target screening and module analysis, and the DAVID database was used for GO function annotation and KEGG signal pathway enrichment analysis. SD rats were orally administered 3.94 g/kg of Huayu Pills decoction for 4 days to prepare medicated serum. HUVEC cells were randomly divided into a control group and an experimental group using a random number table method. The experimental group received intervention with Huayu Pills containing serum for 24 hours, inoculated in matrigel's solidified 48 well plate and HUVEC angiogenesis was observed 3 hours later.Results:130 possible targets of Huayu Pills in the treatment of TNBC were obtained. VEGFA is the core target. The cascade of angiogenesis, hypoxia, and blood coagulation may be the main functions and key signals of Huayu Pills in the treatment of TNBC. In vitro studies have shown that serum containing Huayu Pills can promote the normalization of tumor blood vessels in HUVEC cells.Conclusion:Huayu Pills may promote tumor vascular normalization (angiogenesis, hypoxia, coagulation cascade reactions) through VEGF targets, and can assist immune checkpoint inhibitors in the treatment of TNBC.

Oral Absorption and Labeling Techniques of Traditional Chinese Medicine Polysaccharides： A Review / 中国实验方剂学杂志

Polysaccharides are the important material basis of traditional Chinese medicine（TCM）， and have various pharmacological activities such as immunomodulation， antitumor and anti-aging. Due to the large molecular weight of TCM polysaccharides， their structural analysis and oral absorption mechanism are facing technical challenges， and the current research on their structure-activity relationships has made some breakthroughs， while the research on their oral absorption mechanisms is relatively slow. In-depth study of the oral absorption mechanism of TCM polysaccharides is not only crucial for the interpretation of their action pathways and efficacy in vivo， but also helpful for the interpretation of their pharmacological effects， rational clinical applications and the discovery of new targets. In recent years， the application of fluorescent labeling and isotopic labeling methods has provided new technical means for the oral absorption studies of polysaccharides， which has promoted the development of oral absorption studies of TCM polysaccharides. In this paper， we reviewed the oral absorption pathways and labeling techniques of TCM polysaccharides， and concluded that they can be absorbed orally through transmembrane， cellular bypass， and M-cell-mediated transport， of which transmembrane pathway is the main absorption pathway， and summarized the labeling reactions of four fluorescent labeling and isotopic labeling methods with TCM polysaccharides， which can provide references for evaluating the absorption pathways of TCM polysaccharides， screening active TCM polysaccharides， establishing pharmacodynamic models and comprehensively elucidating the mechanism of TCM polysaccharides.

Magnetic resonance left ventricular hemodynamic analysis: a normal value study of two methods / 中国医师杂志

Objectives:To analyze the consistency of evaluating left ventricular hemodynamics (HDF) based on single plane and multi plane cine sequences of magnetic resonance mitral valve orifice.Methods:A prospective study was conducted on 48 healthy adults, and two methods were used to measure the mitral valve diameter and calculate HDF parameters. The first method was to measure the diameter of the mitral valve opening in the left ventricular three chamber cine sequence; The second method is to measure the mitral valve diameter using cine sequences of two chamber, three chamber, and four chamber hearts, and then take the average value. Paired t-tests were used to compare the differences in HDF measured by two methods, and Pearson correlation coefficient ( r), intra group correlation coefficient ( ICC), and Bland-Altman analysis were used to test the consistency and reproducibility of the two methods. Results:The root mean square (RMS) of longitudinal HDF calculated using single plane and multi plane mitral valve diameters were [(17.28±4.41)% vs (17.21±4.61)%] ( P=0.379) for the entire cardiac cycle, [(21.45±5.54)% vs (21.49±5.68)%] ( P=0.646) for systolic phase, and [(12.78±4.10)% vs (12.54±4.24)%] ( P=0.106) for diastolic phase, respectively. The difference in the calculation results of HDF parameters related to ventricular function was not statistically significant (all P>0.05), and there was good consistency ( r=0.924-0.996, ICC=0.924-0.995). The two HDF parameters related to atrial function were sensitive to the measurement method of mitral valve orifice diameter [RMS of longitudinal HDF during active atrial emptying: (3.26±1.51)% vs (3.32±1.55)%, P=0.006; longitudinal HDF pulse during active atrial emptying: (-2.60±1.28)% vs (-2.76±1.30)%, P<0.001]. Conclusions:The ventricular function related HDF parameters obtained from the analysis of mitral valve orifice diameter using single plane and multi plane methods have good consistency, and can be evaluated using relatively simple single plane methods for left ventricular HDF.

Application and effect of self-management ability-oriented focus solution model in children with nephrotic syndrome / 中国实用护理杂志

Objective:To explore the application effect of self-management ability-oriented focus solution model in children with nephrotic syndrome, and to observe the optimal value of self-management and coping style for children with nephrotic syndrome.Methods:This was a class trial study. A total of 96 children with nephrotic syndrome in Children′s Hospital of Nanjing Medical Universityfrom March 2018 to April 2020 were selected as the research object by convenient sampling method, 48 cases of children from March 2018 to February 2019 for the control group, using conventional psychological counseling, 48 cases of children from March 2019 to April 2020 for the observation group, with self management ability oriented focus solution mode. The self-management and response changes after 2 months of intervention were compared between the two groups.Results:The scores of treatment management, psychological management, diet management and social activity inself-management behavior after intervention were (32.79 ± 2.94), (19.43 ± 1.76), (22.34 ± 1.64), (39.79 ± 2.97) points in the observation group, and (27.42 ± 3.25), (16.79 ± 1.52), (18.46 ± 1.58), (35.28 ± 2.64) points in the control group, the differences were statistically significant ( t values were 7.86-11.80, all P<0.05). The scores of escape, acceptance, negative emotional response, and total score after intervention were (12.41 ± 2.15), (20.41 ± 1.85), (25.89 ± 1.68), (99.37 ± 8.94) points in the observation group, and (11.22 ± 3.04), (19.30 ± 2.09), (24.80 ± 2.10), (95.12 ± 9.02) points in the control group, the differences were statistically significant ( t values were 2.21-2.81, all P<0.05). Conclusions:The self-management-oriented focus solution model for children with nephrotic syndrome can promote the improvement of self-management behavior, and promote the improvement of coping methods in terms of avoidance, acceptance and negative emotional response, which has reference and complementary value for the care of children with nephrotic syndrome.

Research progress on the predicting value of serum markers related to postoperative recurrence in patients with hepatocellular carcinoma after curative resection / 中华检验医学杂志

Patients with surgically resected hepatocellular carcinoma (HCC) have a high recurrence rate within 5 years after surgery. It is therefore essential to explore the risk factors and predictive biomarkers for HCC recurrence and metastasis to identify high-risk patients for HCC recurrence. Serum specimens are readily available and have the advantage of being non-invasive, inexpensive and rapid to detect. Serum markers can dynamically monitor patients′ disease and indicate the status of tumor recurrence. This article summarized the research progress on the predictive value of serum markers associated with recurrence in HCC patients after surgical resection. From the perspectives of common clinical serum markers and liquid biopsy markers, present review aimed to provide some novel ideas for clinicians to assess the risk of HCC recurrence and metastasis in individual patient post-surgical resection of HCC.

A cervical cancer tissue-derived decellularized extracellular matrix scaffold for cervical cancer tissue reconstruction in vitro / 南方医科大学学报

OBJECTIVE@#The prepare decellularized extracellular matrix (ECM) scaffold materials derived from human cervical carcinoma tissues for 3D culture of cervical carcinoma cells.@*METHODS@#Fresh human cervical carcinoma tissues were treated with sodium lauryl ether sulfate (SLES) solution to prepare decellularized ECM scaffolds. The scaffolds were examined for ECM microstructure and residual contents of key ECM components (collagen, glycosaminoglycan, and elastin) and genetic materials by pathological staining and biochemical content analysis. In vitro 3D culture models were established by injecting cultured cervical cancer cells into the prepared ECM scaffolds. The cells in the recellularized scaffolds were compared with those in a conventional 2D culture system for cell behaviors including migration, proliferation and epithelial-mesenchymal transition (EMT) wsing HE staining, immunohistochemical staining and molecular biological technology analysis. Resistance to 5-fluorouracil (5-Fu) of the cells in the two culture systems was tested by analyzing the cell apoptosis rates via flow cytometry.@*RESULTS@#SLES treatment effectively removed cells and genetic materials from human cervical carcinoma tissues but well preserved the microenvironment structure and biological activity of ECM. Compared with the 2D culture system, the 3D culture models significantly promoted proliferation, migration, EMT and 5-Fu resistance of human cervical cancer cells.@*CONCLUSION@#The decellularized ECM scaffolds prepared using human cervical carcinoma tissues provide the basis for construction of in vitro 3D culture models for human cervical cancer cells.

The application of the Chinese version of the European quality of life five-dimension scale in China / 中国医师进修杂志

This paper introduces the composition of the Chinese version of the European quality of life five-dimension scale (EQ-5D), including two different level scales, EQ-5D-3L and EQ-5D-5L, and summarizes the status quo of the application of the above scales. This paper sorts out the utility value sets of the EQ-5D-3L and EQ-5D-5L scales currently developed based on the Chinese population, and provide an important reference for Chinese researchers to choose suitable scales for research on health-related quality of life and health economics cost-utility analysis in the future.

Visualization Analysis of Literatures About Artificial Intelligence in Cancer Research / 肿瘤防治研究

Objective To analyze the literatures about artificial intelligence in cancer research in Web of Science (WOS) core collection database in 2010-2019 and summarize research hot spots and development trends. Methods Through bibliometrics methods and CiteSpace information visualization software, we applied the visual analysis of relevant literature on artificial intelligence in the field of cancer research retrieved from the Web of Science core collection database from 2010 to 2019. Results The number of published articles about artificial intelligence in the field of cancer research had been increasing year by year. The United States ranked first in the number of published articles in this field, the number of citations and cooperation capabilities. Although the number of published articles in China ranked the second, the number of citations was low. The hot spots of artificial intelligence in cancer research were mainly breast cancer and lung cancer. Machine learning, neural network and other methods were used to build models, which were used in basic cancer research, clinical diagnosis, treatment and prognosis prediction. The research frontiers were the methodological research of artificial intelligence, the research on the occurrence and classification of cancer and the research of protein in this field. Conclusion It will effectively promote the development of artificial intelligence in cancer research in China by learning the hot spots and cutting-edge technologies of international research, focusing on international cooperation and cooperation among national institutions and strengthening cross-disciplinary research.

Romosozumab in Postmenopausal Korean Women with Osteoporosis: A Randomized, Double-Blind, Placebo-Controlled Efficacy and Safety Study

Background@#This phase 3 study evaluated the efficacy and safety of 6-month treatment with romosozumab in Korean postmenopausal women with osteoporosis. @*Methods@#Sixty-seven postmenopausal women with osteoporosis (bone mineral density [BMD] T-scores ≤–2.5 at the lumbar spine, total hip, or femoral neck) were randomized (1:1) to receive monthly subcutaneous injections of romosozumab (210 mg; n=34) or placebo (n=33) for 6 months. @*Results@#At month 6, the difference in the least square (LS) mean percent change from baseline in lumbar spine BMD (primary efficacy endpoint) between the romosozumab (9.5%) and placebo (–0.1%) groups was significant (9.6%; 95% confidence interval, 7.6 to 11.5; P<0.001). The difference in the LS mean percent change from baseline was also significant for total hip and femoral neck BMD (secondary efficacy endpoints). After treatment with romosozumab, the percent change from baseline in procollagen type 1 N-terminal propeptide transiently increased at months 1 and 3, while that in C-terminal telopeptide of type 1 collagen showed a sustained decrease. No events of cancer, hypocalcemia, injection site reaction, positively adjudicated atypical femoral fracture or osteonecrosis of the jaw, or positively adjudicated serious cardiovascular adverse events were observed. At month 9, 17.6% and 2.9% of patients in the romosozumab group developed binding and neutralizing antibodies, respectively. @*Conclusion@#Treatment with romosozumab for 6 months was well tolerated and significantly increased lumbar spine, total hip, and femoral neck BMD compared with placebo in Korean postmenopausal women with osteoporosis (ClinicalTrials.gov identifier NCT02791516).

Recommendations for public health protection against flood disaster / 中华预防医学杂志

Flood disaster is one of the most serious natural disasters in the world, and it could pose an inestimable impact on the affected people. Based on existing laws, regulations, and emergency manuals in China, extensive literature review, epidemiological and related protection evidence, and expert consultation, this study analyzed different health risk factors of flood disaster and proposed a multi-stage, multi-population, and multi-phase comprehensive protection measures for the public in the perspective of pre-event prevention, in-event intervention and post-event rescue strategy, which could provide a scientific basis for improving the level of public health protection against the flood disaster and corresponding health outcomes.

Brain activity in using heuristic prototype to solve insightful problems.

When confronted with a real-world problem, heuristic knowledge and experience can guide the solution of a specific technical problem as the key step toward innovation. In particular, a heuristic prototype must be used correctly to cue the technical problem that exists in a particular situation. The present study selected an innovative paradigm and scientific innovation materials to investigate the neural basis of insight induced by heuristic prototypes using event-related functional magnetic resonance imaging (fMRI). The day prior to undergoing fMRI scanning, participants were asked to solve 42 difficult technical problems that scientists might have already encountered but were unknown to the participants. In the subsequent fMRI experiment, the same participants were randomly presented with 84 prototypes classified into two types: related prototypes (RPs), which were useful for solving previously encountered problems, and unrelated prototypes (UPs), which sometimes did not contribute to problem solving. While being scanned, participants were asked to assess whether a prototype is relevant to any of the technical problems. This study comprised two conditions: solving technical problems when presented with a related heuristic prototype and failing to solve technical problems using unrelated heuristic prototypes. The authors assumed that the regions significantly activated by the RP condition, compared with the UP condition, reflected brain activity related to the role of heuristic prototypes in scientific insight. fMRI data showed that the left dorsolateral prefrontal gyrus (left DLFPC, BA9) and the left angular gyrus (left AG, BA39) were more significantly activated when presented with RPs than with UPs. The results suggest that the DLPFC may be involved in the automatic retrieval of technical problems and breaking of mental sets. Moreover, the left AG may be involved in forming novel associations between technical problems and related prototypes.

Anthropometry and the risk of colorectal cancer in males: a prospective cohort study / 中华预防医学杂志

Objective@#To investigate the association between anthropometry and colorectal cancer risk in Chinese males.@*Methods@#Anthropometry and incident colorectal cancer cases were collected on a biennial basis starting in May 2006 among males in Kailuan Cohort (2006-2014). In addition, electronic database of hospitals affiliated to Kailuan Community, Insurance System of Kailuan Community and Tangshan were also searched for supplementary information. Cox proportional hazards regression models and linear models were used to evaluate the association between baseline anthropometry and the risk of colorectal cancer in males.@*Results@#A total of 106 786 males were included and 318 new colorectal cancer cases were identified in the Kailuan male cohort study, with 747 337.60 person-years follow-up by 31 December 2014. The median follow-up time was 7.90 years. Highest quartile waist circumference (≥94.0 cm) or WHtR (≥0.55) had 1.45 (95%CI: 1.05-2.02) and 1.66 (95%CI: 1.15-2.41) higher risk of colorectal cancer when compared with lowest waist circumference (<82.0 cm) or WHtR (<0.48) after adjusting for age, education, smoking, alcohol drinking, sitting time and dust exposure. Subgroup analyses by site indicated that males with BMI ≥26.27 kg/m2, waist circumference ≥94.0 cm or WHtR ≥0.55 had HRs (95%CI) of 2.18(1.27-3.73), 2.20 (1.27-3.78) and 2.42 (1.29-4.56) for colon cancer risk, respectively. Linear models showed the HR of colon cancer and 95%CI would be 1.59 (1.24-2.02) with every 0.1 growth in WHtR.@*Conclusion@#Obesity may be responsible for an increased risk of colorectal cancer in male. Reasonable weight control may be one of the effective measures to prevent colorectal cancer.

Association between total cholesterol and risk of lung cancer incidence in men: a prospective cohort study / 中华流行病学杂志

Objective To assess the association and intensity of baseline TC level with the incidence of lung cancer in men in China.Methods Since May 2006,all the male workers,including the employees and the retirees in Kailuan Group were recruited in the Kailuan male dynamic cohort study.Information about demographics,medical history,anthropometry and TC level were collected at the baseline interview,as well as the information of newly-diagnosed lung cancer cases during the follow-up period.According to guidelines for blood lipids in Chinese adults and the distribution in the population,TC level was classified into five groups as followed:＜ 160,160-,180-,200-and ≥240 mg/dl,with the second quintile group (160-mg/dl) serving as the referent category.Cox proportional hazards regression model and restricted cubic spline (RCS) model were used to evaluate the association and the nonlinear association between baseline TC level and the risk of lung cancer in the men.Results By December 31,2014,for the 109 884 men,a follow up of 763 819.25 person-years was made with a median follow-up period of 7.88 years.During the follow up,808 lung cancer cases were identified.After adjustment for age,education level,income level,smoking status,alcohol consumption level,history of dust exposure,FPG level and BMI,HR (95％CD of lung cancer for men with lower TC level (＜160 mg/dl) and higher TC level (≥240 mg/dl) were 1.34 (1.04-1.72) and 1.45 (1.09-1.92),respectively,compared with men with normal TC level (160-mg/dl).The results didn't change significantly after exclusion of newly diagnosed cancer cases within 2 years of follow up and subjects with the history of hyperlipidemia.Conclusion Our results showed that TC might be associated with higher risk of lung cancer.Men with lower TC level or higher TC level had higher risk for lung cancer.Keep moderate TC level might be one of the effective precaution for the prevention of lung cancer.

Association between total cholesterol and risk of lung cancer incidence in men: a prospective cohort study / 中华流行病学杂志

Objective: To assess the association and intensity of baseline TC level with the incidence of lung cancer in men in China. Methods: Since May 2006, all the male workers, including the employees and the retirees in Kailuan Group were recruited in the Kailuan male dynamic cohort study. Information about demographics, medical history, anthropometry and TC level were collected at the baseline interview, as well as the information of newly-diagnosed lung cancer cases during the follow-up period. According to guidelines for blood lipids in Chinese adults and the distribution in the population, TC level was classified into five groups as followed: <160, 160-, 180-, 200- and ≥240 mg/dl, with the second quintile group (160- mg/dl) serving as the referent category. Cox proportional hazards regression model and restricted cubic spline (RCS) model were used to evaluate the association and the nonlinear association between baseline TC level and the risk of lung cancer in the men. Results: By December 31, 2014, for the 109 884 men, a follow up of 763 819.25 person-years was made with a median follow-up period of 7.88 years. During the follow up, 808 lung cancer cases were identified. After adjustment for age, education level, income level, smoking status, alcohol consumption level, history of dust exposure, FPG level and BMI, HR (95%CI) of lung cancer for men with lower TC level (<160 mg/dl) and higher TC level (≥240 mg/dl) were 1.34 (1.04- 1.72) and 1.45 (1.09-1.92), respectively, compared with men with normal TC level (160- mg/dl). The results didn't change significantly after exclusion of newly diagnosed cancer cases within 2 years of follow up and subjects with the history of hyperlipidemia. Conclusion: Our results showed that TC might be associated with higher risk of lung cancer. Men with lower TC level or higher TC level had higher risk for lung cancer. Keep moderate TC level might be one of the effective precaution for the prevention of lung cancer.

Effect of miR-211 on proliferation and cell cycle related proteins of epithelial ovarian cancer cell line HO8910 / 中国免疫学杂志

Objective:To investigate the relationship between miR-211 and the occurrence of epithelial ovarian cancer,and its influence on the proliferation of ovarian cancer cells. Methods:To analyze the expression miR-211,CDK6 and Cyclin D1 of 30 cases of ovarian cancer and ovarian cancer cell lines,and 30 cases of non-ovarian cancer tissues and the normal ovarian epithelial cells were selected as the control group,and to analyze effects of miR-211 on the proliferation of ovarian cancer cells,as well as the Cyclin D1 and CDK6. Results:miR-211 relative expression level of ovarian cancer tissue was significantly lower than that in the normal group ( P<0. 05). Relative expression level of miR-211 of ovarian cancer cells was significantly lower than that in normal ovarian epithelial cells (P< 0. 05);in epithelial ovarian cancer cell line HO8910,cell number of miR-211 on the 3-day and the 4-day was significantly lower than that of miR-Ctrl group (P<0. 05);relative expression levels of Cyclin D1 and CDK6 in epithelial ovarian cancer were significantly higher than those in normal ovarian epithelial tissues (P<0. 05);miR-211 of epithelial ovarian cancer cell lines significantly inhibited Cyclin D1 and CDK6 expression;in ovarian cancer tissues,Spearman correlation analysis results showed that relative expression levels of miR-211 and Cyclin D1 and CDK6 was negatively correlated ( r=-0. 583, P= 0. 010 ) . Conclusion: miR-211 can inhibit the proliferation of ovarian cancer cells,and inhibit the expression of Cyclin D1 and CDK6;miR-211,Cyclin D1 and CDK6 in ovarian cancer may be involved in the regulation of ovarian cancer.

Mechanism of thiocoraline combination with cisplatin on promoting apoptosis of cervical cancer cell line c4-1 and Hela / 中国免疫学杂志

Objective:To investigate the apoptosis by thiocoraline in combination with cisplatin on cervical cell line c4-1 and Hela and its mechanism. Methods:Different concentrations of thiocoraline and cisplatin alone or in combination used for cultured c4-1 and Hela cells. The inhibition of cell proliferation was detected by MTT assay,and the apoptosis was detected by Hoechst 33342 and AO-EB staining. The expressions of the protein Bax,Bcl2,p53,p21 were detected by Western blot. The cell cycle of c4-1 and Hela cells were detected by Flow cytometry. Results: The cell proliferation activity of c4-1 and HeLa cells were inhibited obviously by different concentrations of thiocoraline and cisplatin alone or in combination. The inhibition effect was in a dose-dependent manner. Meanwhile, when the concentrations of thiocoraline and cisplatin were 10 nmol/L and 4 μmol/L, respectivity the inhibitory effect was most significant (P<0. 05);Hoechst 33342 and AO-EB staining showed that the cell apoptosis effect was more obvious on thiocoraline in combination with cisplatin. The Western blot showed that the expressions of the protein Bax,p53,p21 were significantly up-regulated, and the Bcl2 was significantly down-regulated. Conclusion:Thiocoraline combination with cisplatin could up-regulate the expression of the protein Bax,p53,p21 and down-regulation the expression of Bcl2 to inhibition the proliferation and promotion apoptosis of cervical cell line c4-1 and Hela.

Effectiveness of tyrosine kinase inhibitors against non-small cell lung cancer patients with postoperative recurrence harboring uncommon EGFR mutations / 中华肿瘤杂志

Objective@#To investigate the efficacy of tyrosine kinase inhibitor (TKI) treatment on non-small cell lung cancer (NSCLC) patients with postoperative recurrence who harbored uncommon EGFR mutations, and discuss the relationship between TKI treatment and prognosis.@*Methods@#A total of 39 relapsed NSCLC patients after surgery with EGFR uncommon mutations who were detected at Cancer Hospital, Chinese Academy of Medical Sciences between January 1999 and December 2013 were retrospectively analyzed in this study. Twenty patients were treated with EGFR-TKI after recurrence and 19 cases were not. The clinical characteristics of patients with EGFR uncommon mutations were evaluated, and the prognosis of TKI-treatment group and non-TKI treatment group was compared.@*Results@#In 39 relapsed NSCLC patients with EGFR uncommon mutations, insertion mutations and point mutations were included. The highest frequency of EGFR uncommon mutation happened in exon 20 (20/39, 51.3%). A total of 13 uncommon point mutations were detected in exon 18, 20 and 21. The most frequent rare point mutations located in exon 21, and there were 7 different point mutation sites in exon 21. G719S/C/A mutation in exon 18 was the most common type of point mutation (14/25, 56.0%). Survival after postoperative recurrence in TKI treatment group was obviously better than that in non-TKI treatment group, the median time after recurrence were 44 months and 23 months, respectively (P=0.044). However, the postoperative overall survival showed no differences between two groups (48 months vs 43 months, P=0.129).@*Conclusion@#NSCLC patients with postoperative recurrence who harbored rare EGFR mutations should be treated with TKI agent.

Effects of naringin on growth and COX-2 expressions in human cervical cancer HeLa cell line / 中国免疫学杂志

Objective: To study the effect of naringin on human cervical cancer HeLa cell line and its related mechanism.Methods:HeLa cells were cultured in vitro in the presence of different concentration naringin .The method of MTT was used to detect the growth of HeLa cell in 24 h, 48 , 72 h.Apoptotic cells were observed by Hoechst 33258 staining.The protein expressions of COX-2 in SCL-12 cells were analyzed by Western blot .Results: The inhibition ratio of the cell growth in middle dose group and high dose group significantly higher than control group ( P0.05).The apoptosis ratio of the cell growth in middle dose group and high dose group significantly higher than control group( P0.05 ) .The expression of COX-2 was significantly decreased in middle dose group and high dose group .Conclusion: Naringin plays a central role in the apoptosis of the human cervical cancer HeLa cell line ,which may be related to the expression of COX-2.

CD177+ neutrophils and its correlation with mucosal impairment in patients with inflammatory bowel disease / 中华消化内镜杂志

Objective To investigate the percentage of CD177+ neutrophils in peripheral blood and inflamed mucosa of the intestine in patients with inflammatory bowel disease(IBD)and in healthy controls, and to investigate the correlation between CD177+ neutrophils and mucosal impairment as well as clinical characteristics. Methods A total of 54 patients with ulcerative colitis(UC),62 patients with Crohn disease (CD),and 48 healthy controls were enrolled. SYBR-green Realtime PCR was applied to determine the ex-pression proportion of CD177+ neutrophils in peripheral blood and in inflamed intestinal mucosa to investigate the correlation between CD177+ neutrophils and endoscopic and clinical disease activity. Results In periph-eral blood and inflamed mucosa of the intestine,the expression of CD177+ neutrophils was significantly higher than that in health controls or patients in remission stage(P< 0. 05),with higher proportion( P<0. 05). The expression of CD177+ neutrophils in peripheral blood and inflamed mucosa of the intestine was positively correlated with both endoscopic disease activity index and clinical severity of the disease( P <0. 05). Conclusion CD177+ neutrophils increase in peripheral blood and intestinal mucosa of patients with IBD. The percentage of CD177+ neutrophils is a favorable marker for both endoscopic disease activity and clinical severity of the disease. CD177+ neutrophils may play a vital role in the immune response in the intes-tinal mucosa,as a new subset of effector neutrophils.

Effect of Dysbiosis of Intestinal Microbiota in Inflammatory Bowel Disease / 胃肠病学

Human intestinal microbial flora has complex interaction with intestinal mucosal immune system,which maintains intestinal homeostasis. Inflammatory bowel disease( IBD)is a chronic and nonspecific intestinal inflammatory disease,the etiology and pathogenic mechanisms have not yet been fully clarified. Dysbiosis of intestinal microbiota is an important factor in the pathogenesis of IBD,and modulation of intestinal microbiota can be one of the measures for treatment of IBD. This article reviewed the dysbiosis of intestinal microbiota in IBD.